Two novel secreted ferritins involved in immune defense of Chinese mitten crab Eriocheir sinensis

doi:10.1016/j.fsi.2009.12.018

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	Two novel secreted ferritins involved in immune defense of Chinese mitten crab Eriocheir sinensis
	Kong, Pengfei 1,2; Wang, Lingling 1; Zhang, Huan 1,2; Zhou, Zhi 1,2; Qiu, Limei 1; Gai, Yunchao 1; Song, Linsheng 1
	2010-04-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	28 期号:4 页码:604-612
文章类型	Article
摘要	As a principal extracellular iron storage molecule, secreted ferritin plays an important role in the iron-withholding strategy of innate immunity. In this study, two novel secreted ferritins were identified from Chinese mitten crab Eriocheir sinensis (designated as EsFer-1 and EsFer-2) by rapid amplification of cDNA ends (RACE) approaches and expressed sequence tag (EST) analysis. The full-length cDNAs of EsFer-1 and EsFer-2 were of 1278 and 1595 bp, respectively, both containing a putative iron response element (IRE) in their 5' UTRs and multiple A + U-destabilizing elements (TATT or ATTTA) in their 3' UTRs. The ORFs of these two crab ferritin cDNAs were of 639 and 663 bp, respectively, encoding two peptides of 212 and 220 amino acid residues each with a signal peptide and typical structures of ferritins such as four long alpha-helices, one short alpha-helix and an L-loop. EsFer-2 exhibited higher similarity with the H-ferritins from both invertebrates and vertebrates, while EsFer-1 was closer matched to L-ferritins. The eight amino acid residues identified as metal binding sites in vertebrate H-ferritins were conserved in EsFer-2 (Glu53, Tyr60, Glu87, Glu88, His91, Glu146, Glu177 and Gln178), but none of them was observed in EsFer-1. By fluorescent quantitative real-time PCR, mRNA transcripts of EsFer-1 and EsFer-2 were mainly detected in muscle, hepatopancreas and gill, and also marginally detectable in gonad, heart and hemocytes. After the crabs were challenged by bacteria Listonella anguillarum, the transcriptional levels of both EsFer-1 and EsFer-2 in hemocytes were up-regulated twice. In the first up-regulation, the mRNA relative expression levels of both EsFer-1 and EsFer-2 reached peak at 3 h post-challenge, while in the second up-regulation, they did not reach the highest point within the experiment duration. After the fungi Pichia pastoris GS115 challenge, there was only one transcriptional level peak of both the two ferritins, appearing at 6 h post-challenge. These results suggest that secreted EsFer-1 and EsFer-2 are crucial proteins in the iron-withholding defense system, and play important roles in the innate immune responses in crabs. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Secreted Ferritin Iron-withholding Strategy Innate Immunity Metal Binding Site Pathogen Infection Eriocheir Sinensis
DOI	10.1016/j.fsi.2009.12.018
收录类别	SCI
语种	英语
WOS记录号	WOS:000276935700013
引用统计	被引频次：46[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32812
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Kong, Pengfei,Wang, Lingling,Zhang, Huan,et al. Two novel secreted ferritins involved in immune defense of Chinese mitten crab Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(4):604-612.
APA	Kong, Pengfei.,Wang, Lingling.,Zhang, Huan.,Zhou, Zhi.,Qiu, Limei.,...&Song, Linsheng.(2010).Two novel secreted ferritins involved in immune defense of Chinese mitten crab Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,28(4),604-612.
MLA	Kong, Pengfei,et al."Two novel secreted ferritins involved in immune defense of Chinese mitten crab Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 28.4(2010):604-612.

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