Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Molecular characterization and expression analysis of chitinase (Fcchi-3) from Chinese shrimp, Fenneropenaeus chinensis | |
Zhang, Jiquan1; Sun, Yuying2; Li, Fuhua1; Huang, Bingxin1; Xiang, Jianhai1
| |
| 2010-04-01 | |
| 发表期刊 | MOLECULAR BIOLOGY REPORTS
 |
| 卷号 | 37期号:4页码:1913-1921 |
| 文章类型 | Article |
| 摘要 | Complementary DNA (cDNA) and genomic DNA, including flanking regions of the chitinase gene (Fcchi-3) of Fenneropenaeus chinensis, were cloned and sequenced. Fcchi-3 was found to have 92.0 and 91.4% identity at the cDNA level to that of Litopenaeus vannamei and Marsupenaeus japonicus, respectively. The predicted amino acid sequence had an overall similarity with a comparable region of L. vannamei (96.8%) and M. japonicus (93.4%). Based on the cDNA sequence, the genomic structure of the gene was characterized. Sequence analysis revealed that the Fcchi-3 gene was composed of seven exons with 411, 252, 186, 132, 171, 117 and 135 bp and six introns with 232, 196, 121, 90, 159 and 157 bp. Analysis by RT-PCR revealed that Fcchi-3 was a hepatopancreas specific gene. Semi-quantitative RT-PCR analysis revealed that Fcchi-3 transcript was down-regulated significantly in response to the challenge of WSSV at 5 h post-injection and then came back to normal level at 37 h. A fusion protein containing Fcchi-3 was produced and the purified recombinant protein exhibited similar biological function. The result of identification through LC-ESI-MS showed that three peptide fragments (-MAADPVLR-, -ATIDPAYNVPELSK- and -AILAVGGWNEGSPK-) of the recombinant protein were identical to the corresponding sequence of L. vannamei chitinase-3. The recombinant Fcchi-3 could degrade the colloid chitin confirming that the recombinant protein is actually the chitinase. |
| 关键词 | Fenneropenaeus Chinensis Chitinase Recombinant Expression Lc-esi-ms |
| DOI | 10.1007/s11033-009-9633-0 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275161700029 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32810 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Jiquan,Sun, Yuying,Li, Fuhua,et al. Molecular characterization and expression analysis of chitinase (Fcchi-3) from Chinese shrimp, Fenneropenaeus chinensis[J]. MOLECULAR BIOLOGY REPORTS,2010,37(4):1913-1921. |
| APA | Zhang, Jiquan,Sun, Yuying,Li, Fuhua,Huang, Bingxin,&Xiang, Jianhai.(2010).Molecular characterization and expression analysis of chitinase (Fcchi-3) from Chinese shrimp, Fenneropenaeus chinensis.MOLECULAR BIOLOGY REPORTS,37(4),1913-1921. |
| MLA | Zhang, Jiquan,et al."Molecular characterization and expression analysis of chitinase (Fcchi-3) from Chinese shrimp, Fenneropenaeus chinensis".MOLECULAR BIOLOGY REPORTS 37.4(2010):1913-1921. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Molecular characteri（578KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论