Molecular characterization and expression of a crustin-like gene from Chinese mitten crab, Eriocheir sinensis

doi:10.1016/j.dci.2010.02.001

IOCAS-IR > 实验海洋生物学重点实验室

	Molecular characterization and expression of a crustin-like gene from Chinese mitten crab, Eriocheir sinensis
	Mu, Changkao 1,2; Zheng, Peilin 1; Zhao, Jianmin 1; Wang, Lingling 1; Zhang, Huan 1; Qiu, Limei 1; Gai, Yunchao 1; Song, Linsheng 1
	2010-07-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	34 期号:7 页码:734-740
文章类型	Article
摘要	Crustin is a cysteine-rich antibacterial peptide which widely distributes within decapod crustaceans. In the present study, the cDNA encoding crustin-like peptide (designated CrusEs) was cloned from Chinese mitten crab Eriocheir sinensis by using rapid amplification of cDNA ends (RACE) approaches and expressed sequence tag analysis. The full-length cDNA of CrusEs was of 796 bp, containing a 5' untranslated region (UTR) of 214 bp, a 3' UTR of 267 bp with a poly(A) tail, and an open reading frame (ORF) of 315 bp encoding a polypeptide of 104 amino acids including a putative signal peptide of 21 amino acids. A WAP domain and the consensus framework existing in class I crustins were both identified in CrusEs, suggesting that CrusEs is a new member of type I crustins. Quantitative real-time RT-PCR was employed to examine the expression of CrusEs, and its mRNA transcript was mainly detected in haemocytes and gill. The CrusEs mRNA transcript in haemocytes was down-regulated after the challenge with Gram-positive bacteria Micrococcus luteus, while Gram-negative bacteria Listonella anguillarum did not induce significant variation of CrusEs mRNA. The mature peptide of CrusEs was cloned into pET-21a(+) with a C-terminal hexa-histidine tag fused in-frame, and expressed in Escherichia coli, and the recombinant CrusEs inhibited the growth of Gram-positive bacteria with low MIC. The results indicate that CrusEs is a potent antibacterial protein against Gram-positive bacteria infection, and it may play an important role in innate immune response of E. sinensis. (C) 2010 Elsevier Ltd. All rights reserved.
关键词	Eriocheir Sinensis Crustin Wap Domain Recombinant Expression Antibacterial Activity Minimal Inhibitory Concentration (Mic)
DOI	10.1016/j.dci.2010.02.001
收录类别	SCI
语种	英语
WOS记录号	WOS:000277891600006
引用统计	被引频次：53[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32793
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Mu, Changkao,Zheng, Peilin,Zhao, Jianmin,et al. Molecular characterization and expression of a crustin-like gene from Chinese mitten crab, Eriocheir sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2010,34(7):734-740.
APA	Mu, Changkao.,Zheng, Peilin.,Zhao, Jianmin.,Wang, Lingling.,Zhang, Huan.,...&Song, Linsheng.(2010).Molecular characterization and expression of a crustin-like gene from Chinese mitten crab, Eriocheir sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,34(7),734-740.
MLA	Mu, Changkao,et al."Molecular characterization and expression of a crustin-like gene from Chinese mitten crab, Eriocheir sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 34.7(2010):734-740.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Molecular characteri（571KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览