Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Phylogenetic analysis and screening of antimicrobial and cytotoxic activities of moderately halophilic bacteria isolated from the Weihai Solar Saltern (China) | |
Chen, Lei1,2,3 ; Wang, Guangyu2; Bu, Tong1,3; Zhang, Yunbin2; Wang, Yixin4; Liu, Ming1; Lin, Xiukun1
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| 2010-05-01 | |
| 发表期刊 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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| 卷号 | 26期号:5页码:879-888 |
| 文章类型 | Article |
| 摘要 | A total of 45 moderately halophilic bacteria was isolated from sediment and saline water collected from the Weihai Solar Saltern (China). The phylogenetic position of all the isolated strains was determined by 16S rRNA sequencing. The halophilic strains were tested for their antimicrobial activity. Cytotoxicity assay was performed to determine which of the halophilic strains could inhibit proliferation of human hepatocellular carcinoma Bel 7402 cells. Our results showed that all of the isolated 45 strains displayed moderately halophilic characteristics. Phylogenetic analysis indicated that 17 of the isolated strains were related to the phylum Firmicutes and belonged to four genera, Bacillus, Halobacillus, Planococcus and Salinicoccus. The other strains identified as genus of Halomonas belonged to phylum gamma-Proteobacteria. Most of the halophilic bacterial strains showed potent activities against Gram-positive bacteria, human pathogenic fungi and plant pathogenic fungi. In addition, the crude extracts from 14 halophilic bacterial strains showed cytotoxic activity against tumor cells Bel 7402, and five of them showed remarkable activities with IC(50) less than 40 mu g ml(-1). Our results suggest that the moderately halophilic bacteria may be developed as promising sources for the discovery of novel bioactive substances. |
| 关键词 | Moderately Halophilic Bacteria Phylogenetic Analysis Antimicrobial Activity Cytotoxic Activity Solar Saltern |
| DOI | 10.1007/s11274-009-0247-4 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276497600015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32784 |
| 专题 | 实验海洋生物学重点实验室 海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Harbin Inst Technol, Coll Marine Sci, Weihai 264209, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Chen, Lei,Wang, Guangyu,Bu, Tong,et al. Phylogenetic analysis and screening of antimicrobial and cytotoxic activities of moderately halophilic bacteria isolated from the Weihai Solar Saltern (China)[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2010,26(5):879-888. |
| APA | Chen, Lei.,Wang, Guangyu.,Bu, Tong.,Zhang, Yunbin.,Wang, Yixin.,...&Lin, Xiukun.(2010).Phylogenetic analysis and screening of antimicrobial and cytotoxic activities of moderately halophilic bacteria isolated from the Weihai Solar Saltern (China).WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,26(5),879-888. |
| MLA | Chen, Lei,et al."Phylogenetic analysis and screening of antimicrobial and cytotoxic activities of moderately halophilic bacteria isolated from the Weihai Solar Saltern (China)".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 26.5(2010):879-888. |
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