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| Recovery of recombinant zebrafish p53 protein from inclusion bodies and its binding activity to p53 mRNA in vitro | |
| Zhao, Xiangzhong1,2; Liu, Ming1; Wu, Ning1; Ding, Lili1; Liu, Haizhou1; Lin, Xiukun1 | |
| 2010-08-01 | |
| 发表期刊 | PROTEIN EXPRESSION AND PURIFICATION
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| 卷号 | 72期号:2页码:262-266 |
| 文章类型 | Article |
| 摘要 | p53 protein is an important regulation factor that can bind to p53 mRNA to regulate its translation in human and murine. To determine if a similar interaction exists in zebrafish and if the interaction affects zebrafish development, we cloned and expressed p53 protein from zebrafish in Escherichia coli. Soluble p53 protein with high purity was successfully obtained using the optimized renaturation approach. Results of a UV-crosslinking experiment and immunoprecipitation:RT-PCR analysis confirmed that the purified p53 protein could bind specifically to its cognate mRNA. Our results suggest that selecting a suitable buffer is important for renaturing p53 protein from inclusion bodies. We also demonstrated a specific interaction between p53 and it own RNA in zebrafish. Measurement of the binding activity may be a useful approach for identifying the activity of recombinant p53 protein in vitro. (C) 2010 Elsevier Inc. All rights reserved. |
| 关键词 | P53 Zebrafish Rna Binding Protein Protein Expression Inclusion Body |
| DOI | 10.1016/j.pep.2010.03.028 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278193200017 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32772 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, Xiangzhong,Liu, Ming,Wu, Ning,et al. Recovery of recombinant zebrafish p53 protein from inclusion bodies and its binding activity to p53 mRNA in vitro[J]. PROTEIN EXPRESSION AND PURIFICATION,2010,72(2):262-266. |
| APA | Zhao, Xiangzhong,Liu, Ming,Wu, Ning,Ding, Lili,Liu, Haizhou,&Lin, Xiukun.(2010).Recovery of recombinant zebrafish p53 protein from inclusion bodies and its binding activity to p53 mRNA in vitro.PROTEIN EXPRESSION AND PURIFICATION,72(2),262-266. |
| MLA | Zhao, Xiangzhong,et al."Recovery of recombinant zebrafish p53 protein from inclusion bodies and its binding activity to p53 mRNA in vitro".PROTEIN EXPRESSION AND PURIFICATION 72.2(2010):262-266. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Recovery of recombin（306KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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