Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology

doi:10.1016/S0093-691X(03)00097-9

IOCAS-IR > 实验海洋生物学重点实验室

	Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology
	Zhang, YZ ; Zhang, SC ; Liu, XZ ; Xu, YY ; Wang, CL ; Sawant, MS ; Li, J; Chen, SL
	2003-09-15
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	60 期号:5 页码:989-996
文章类型	Article
摘要	A simple and convenient protocol for the cryopreservation of the flounder (Paralichthys olivaceus) sperm was established for "on the spot" cryopreservation of large quantities of semen. The use of three cryoprotectants, dimethyl sulphoxide (DMSO), glycerol (Gly) and methanol was tested in the method. The percentage of motile sperm present in semen after it had been frozen and thawed in the presence of DMSO, Gly or methanol was 60.5 +/- 3.6, 79.17 +/- 4.5 and 13.25 +/- 4.7%, respectively. The fertilization rates of this sperm were 67.06 +/- 15.1, 76.20 +/- 10.0 and 44.93 +/- 22.6%, while the hatching rates of eggs fertilized with this sperm were 37.40 +/- 8.3, 48.18 +/- 25.7 and 23.35 +/- 10.8%, respectively. It was found that Gly and DMSO were better cryoprotectants than methanol, with Gly giving the best overall results. Under scanning electron microscopy, it could be seen that while the majority of the frozen-thawed sperm remained morphologically normal, some exhibited lost or dilated mitochondria, swollen mid-pieces, broken tails, or damaged cell membrane, which probably caused the decrease in motility and fertility of the frozen-thawed sperm. (C) 2003 Elsevier Science Inc. All rights reserved.; A simple and convenient protocol for the cryopreservation of the flounder (Paralichthys olivaceus) sperm was established for "on the spot" cryopreservation of large quantities of semen. The use of three cryoprotectants, dimethyl sulphoxide (DMSO), glycerol (Gly) and methanol was tested in the method. The percentage of motile sperm present in semen after it had been frozen and thawed in the presence of DMSO, Gly or methanol was 60.5 +/- 3.6, 79.17 +/- 4.5 and 13.25 +/- 4.7%, respectively. The fertilization rates of this sperm were 67.06 +/- 15.1, 76.20 +/- 10.0 and 44.93 +/- 22.6%, while the hatching rates of eggs fertilized with this sperm were 37.40 +/- 8.3, 48.18 +/- 25.7 and 23.35 +/- 10.8%, respectively. It was found that Gly and DMSO were better cryoprotectants than methanol, with Gly giving the best overall results. Under scanning electron microscopy, it could be seen that while the majority of the frozen-thawed sperm remained morphologically normal, some exhibited lost or dilated mitochondria, swollen mid-pieces, broken tails, or damaged cell membrane, which probably caused the decrease in motility and fertility of the frozen-thawed sperm. (C) 2003 Elsevier Science Inc. All rights reserved.
关键词	Flounder Paralichthys Olivaceus Sperm Cryopreservation Glycerol Dimethyl Sulphoxide Methanol
学科领域	Reproductive Biology ; Veterinary Sciences
DOI	10.1016/S0093-691X(03)00097-9
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000185216600018
引用统计	被引频次：75[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3274
专题	实验海洋生物学重点实验室
作者单位	1.Ocean Univ Qingdao, Dept Marine Biol, Qingdao 266003, Peoples R China 2.Liaocheng Univ, Dept Life Sci, Liaocheng 252059, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, YZ,Zhang, SC,Liu, XZ,et al. Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology[J]. THERIOGENOLOGY,2003,60(5):989-996.
APA	Zhang, YZ.,Zhang, SC.,Liu, XZ.,Xu, YY.,Wang, CL.,...&Chen, SL.(2003).Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology.THERIOGENOLOGY,60(5),989-996.
MLA	Zhang, YZ,et al."Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology".THERIOGENOLOGY 60.5(2003):989-996.

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