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| Determination of Dissolved Free Putrescine, Spermidine and Spermine in Seawater by High Performance Liquid Chromatography | |
| Fu Min1,2; Zhao Wei-Hong1; Miao Hui1; Lue Gui-Cai3 | |
| 2010-10-01 | |
| 发表期刊 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 卷号 | 38期号:10页码:1445-1449 |
| 文章类型 | Article |
| 摘要 | A highly sensitive and accurate reversed phase high performance liquid chromatographic (RP-HPLC) method was developed to determine dissolved free polyamines (putrescine, spermidine and spermine) in seawater. This method is based on pre-column derivatization with dansyl chloride (DNS-Cl). The derivatives were separated on a ODS column (150 mm x 4. 6 mm i. d., 5 m particle size, Agilent). The gradient elution was performed with two mobile phases A (acetonitrile) and B (ammonium acetate) at a flow rate of 1.0 mL/min. The column eluate was monitored by fluorescence detection (lambda(ex) = 340 nm; lambda(em) = 515 nm). The detection limits for putrescine, spermidine and spermine were 9.6 x 10(-11), 2. 8 x 10(-10) and 1.0 x 10(-10) mol/L respectively. The reliability of the method was satisfactory in terms of linearity (R > 0. 99), precision(relative standard deviation below 3. 6%) and recovery (from 84.9% to 110. 9%). This analytical technique requires neither preliminary extraction, nor long multi-step procedures. This method can be applied to the determination of dissolved free polyamines (putrescine, spermidine and spermine) in seawater. |
| 关键词 | High Performance Liquid Chromatography Seawater Putrescine Spermidine Spermine |
| DOI | 10.3724/SP.J.1096.2010.01445 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000283904400012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32741 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Ocean Univ China, Key Lab Marine Chem Theory & Technol, Minist Educ, Qingdao 266100, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Fu Min,Zhao Wei-Hong,Miao Hui,et al. Determination of Dissolved Free Putrescine, Spermidine and Spermine in Seawater by High Performance Liquid Chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38(10):1445-1449. |
| APA | Fu Min,Zhao Wei-Hong,Miao Hui,&Lue Gui-Cai.(2010).Determination of Dissolved Free Putrescine, Spermidine and Spermine in Seawater by High Performance Liquid Chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,38(10),1445-1449. |
| MLA | Fu Min,et al."Determination of Dissolved Free Putrescine, Spermidine and Spermine in Seawater by High Performance Liquid Chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 38.10(2010):1445-1449. |
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