Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development | |
Wang, Bo; Mo, Zhao Lan; Xiao, Peng; Li, Jie ; Zou, Yu Xia; Hao, Bin; Li, Gui Yang
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| 2010-11-01 | |
| 发表期刊 | MARINE BIOTECHNOLOGY
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| 卷号 | 12期号:6页码:678-685 |
| 文章类型 | Article |
| 摘要 | Edwardsiella tarda has a type III secretion system (T3SS) essential for pathogenesis. EseD, together with EseB and EseC, has been suggested to form a putative T3SS translocon complex, although its further function is unclear. To investigate the physiological role of EseD, a mutant strain of E. tarda was constructed with an in-frame deletion of the entire eseD gene. One finding was that the a dagger eseD mutant decreased the secretion levels of EseC and EseB proteins. Additionally, the a dagger eseD mutant showed attenuated swarming and contact-hemolysis abilities. However, the a dagger eseD mutant showed increased biofilm formation. Complementation of the mutant strain with eseD restored these phenotypes to those similar to the wild-type strain. Furthermore, infection experiments in fish showed that the a dagger eseD mutant exhibited slower proliferation and a tenfold decrease in virulence in fish. These results indicate a specific role of EseD in the pathogenesis of E. tarda. Finally, recombinant EseD protein elicited high antibody titers in immunized fish and various levels of protection against lethal challenge with the wild-type strain. These results indicate that EseD protein may be a candidate antigen for development of a subunit vaccine against Edwardsiellosis. |
| 关键词 | E. Tarda Esed Mutant Pathogenesis Recombinant Esed Protective Immunity |
| DOI | 10.1007/s10126-009-9255-5 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284599000006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32740 |
| 专题 | 实验海洋生物学重点实验室 海洋生物分类与系统演化实验室 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Bo,Mo, Zhao Lan,Xiao, Peng,et al. EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development[J]. MARINE BIOTECHNOLOGY,2010,12(6):678-685. |
| APA | Wang, Bo.,Mo, Zhao Lan.,Xiao, Peng.,Li, Jie.,Zou, Yu Xia.,...&Li, Gui Yang.(2010).EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development.MARINE BIOTECHNOLOGY,12(6),678-685. |
| MLA | Wang, Bo,et al."EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development".MARINE BIOTECHNOLOGY 12.6(2010):678-685. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| EseD, a Putative T3S（308KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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