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| Cloning and expression profiles of two isoforms of a CHH-like gene specifically expressed in male Chinese shrimp, Fenneropenaeus chinensis | |
Li, Shihao1,2; Li, Fuhua1; Wang, Bing1; Xie, Yusu1,2; Wen, Rong1,2; Xiang, Jianhai1
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| 2010-06-01 | |
| 发表期刊 | GENERAL AND COMPARATIVE ENDOCRINOLOGY
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| 卷号 | 167期号:2页码:308-316 |
| 文章类型 | Article |
| 摘要 | Two full-length cDNA sequences (Fc-CHH1, Fc-CHH2) encoding a crustacean hyperglycemic hormone (CHH) precursor homolog and their DNA sequences were cloned from Chinese shrimp Fenneropenaeus chinensis. The deduced amino acid sequences of them are predicted to contain a signal peptide and a mature peptide. The mature peptides of Fc-CHH1 and Fc-CHH2 shared 78% identity, but they showed low identities (less than 40%) to CHH peptides from other species. Both Fc-CHH1 and Fc-CHH2 proteins contain six highly conserved cysteine residues which are characteristic of the CHH family peptides. The transcripts of Fc-CHH1 and Fc-CHH2 were shown to be specifically present in the spermatophore sac of mature male Chinese shrimp through reverse transcription-polymerase chain reaction (RT-PCR) detection. The transcripts of Fc-CHH1 and Fc-CHH2 begin to appear at the immature stage (115 days after the first post-larvae stage) when the spermatophore sac was first observed to be appeared. In situ hybridization analyses showed that Fc-CHH1 and Fc-CHH2 transcripts located at the epithelial cells in the internal wall of the spermatophore sac. In the cloned DNA sequences of Fc-CHH1 and Fc-CHH2, the predicted transcription factor binding sites in the 5' flanking sequences are different from those previously reported for CHH family genes of crustacean. To our knowledge, these are novel CHH-like genes expressed specifically in male shrimp. Their function needs to be further investigated. (C) 2010 Elsevier Inc. All rights reserved. |
| 关键词 | Crustacean Hyperglycemic Hormone (Chh) Cloning Expression In Situ Hybridization Spermatophore Sac Fenneropenaeus Chinensis |
| DOI | 10.1016/j.ygcen.2010.03.028 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278172500014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32717 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Shihao,Li, Fuhua,Wang, Bing,et al. Cloning and expression profiles of two isoforms of a CHH-like gene specifically expressed in male Chinese shrimp, Fenneropenaeus chinensis[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2010,167(2):308-316. |
| APA | Li, Shihao,Li, Fuhua,Wang, Bing,Xie, Yusu,Wen, Rong,&Xiang, Jianhai.(2010).Cloning and expression profiles of two isoforms of a CHH-like gene specifically expressed in male Chinese shrimp, Fenneropenaeus chinensis.GENERAL AND COMPARATIVE ENDOCRINOLOGY,167(2),308-316. |
| MLA | Li, Shihao,et al."Cloning and expression profiles of two isoforms of a CHH-like gene specifically expressed in male Chinese shrimp, Fenneropenaeus chinensis".GENERAL AND COMPARATIVE ENDOCRINOLOGY 167.2(2010):308-316. |
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