Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Chromosomal localization of 5S rDNA in Chinese shrimp (Fenneropenaeus chinensis): a chromosome-specific marker for chromosome identification | |
Huan Pin1,2 ; Zhang Xiaojun1; Li Fuhua1; Zhao Cui1,2; Zhang Chengsong1,2; Xiang Jianhai1
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| 2010-03-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 卷号 | 28期号:2页码:233-238 |
| 文章类型 | Article |
| 摘要 | Chinese shrimp (Fenneropenaeus chinensis) is an economically important aquaculture species in China. However, cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze. In this study, fluorescence in-situ hybridization (FISH) was used to identify the chromosomes of F. chinensis. The 5S ribosomal RNA gene (rDNA) of F. chinensis was isolated, cloned and then used as a hybridization probe. The results show that the 5S rDNA was located on one pair of homologous chromosomes in F. chinensis. In addition, triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method. It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in F. chinensis. The successful application of FISH in F. chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes of penaeid shrimps. |
| 关键词 | Fenneropenaeus Chinensis 5s Rdna Chromosomal Localization Fluorescence In-situ Hybridization (Fish) |
| DOI | 10.1007/s00343-010-9278-9 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275781400005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32715 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Huan Pin,Zhang Xiaojun,Li Fuhua,et al. Chromosomal localization of 5S rDNA in Chinese shrimp (Fenneropenaeus chinensis): a chromosome-specific marker for chromosome identification[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(2):233-238. |
| APA | Huan Pin,Zhang Xiaojun,Li Fuhua,Zhao Cui,Zhang Chengsong,&Xiang Jianhai.(2010).Chromosomal localization of 5S rDNA in Chinese shrimp (Fenneropenaeus chinensis): a chromosome-specific marker for chromosome identification.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(2),233-238. |
| MLA | Huan Pin,et al."Chromosomal localization of 5S rDNA in Chinese shrimp (Fenneropenaeus chinensis): a chromosome-specific marker for chromosome identification".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.2(2010):233-238. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Chromosomal localiza（870KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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