Detection of the neurotoxin BMAA within cyanobacteria isolated from freshwater in China

doi:10.1016/j.toxicon.2009.09.023

	Detection of the neurotoxin BMAA within cyanobacteria isolated from freshwater in China
	Li, Aifeng 2; Tian, Zhijia 2; Li, Jing2 ; Yu, Rencheng 3; Banack, Sandra Anne 1; Wang, Zhenyu 2
	2010-05-01
发表期刊	TOXICON
卷号	55 期号:5 页码:947-953
文章类型	Article
摘要	The cyanobacterial neurotoxin, beta-N-methylamino-L-alanine (BMAA), has been suggested as an important environmental factor for neurodegenerative disease such as amyotrophic lateral sclerosis- Parkinsonism dementia complex (ALS/PDC) in Guam. BMAA was detected within the majority of cyanobacterial isolates surveyed in both free and symbiotic cyanobacteria, living in freshwater as well as marine environments. In this study, we report two methods using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS) each coupled with a different type of hydrophilic interaction liquid chromatography (HILIC) column to detect BMAA. A third method using AQC-derivatized BMAA was also used for comparison. Axenic cultures of Microcystis aeruginosa and Nostoc sp. isolated from Chinese freshwater were analyzed for both free and protein-bound BMAA at the exponential growth stage. Cultures of two strains of M. aeruginosa collected at four growth stages were also analyzed for the presence of BMAA. BMAA was detected in the Nostoc sp. at very low concentrations (<0.07 pmoles on column) only when precolumn AQC derivatization was used. No BMAA was detected in the Chinese derived axenic cultures of Microcystis; detection limits for the LC-ESI-MS and LC-ESI-MS/MS without precolumn derivatization were 10 ng and 2 pg BMAA on column, respectively. We suggest that cyanobacteria grown under some culture conditions may be relatively free of BMAA. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Beta-n-methylamino-l-alanine (Bmaa) Neurotoxin Cyanobacteria Nostoc Sp. Microcystis Aeruginosa Liquid Chromatography-electrospray Ionization-mass Spectrometry/mass Spectrometry (Lc-esi-ms/ms)
DOI	10.1016/j.toxicon.2009.09.023
收录类别	SCI
语种	英语
WOS记录号	WOS:000276436400006
引用统计	被引频次：64[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32707
专题	海洋生态与环境科学重点实验室实验海洋生物学重点实验室
作者单位	1.Inst Ethnomed, Jackson, WY 83001 USA 2.Ocean Univ China, Key Lab Marine Environm Sci & Ecol, Qingdao 266100, Peoples R China 3.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, Aifeng,Tian, Zhijia,Li, Jing,et al. Detection of the neurotoxin BMAA within cyanobacteria isolated from freshwater in China[J]. TOXICON,2010,55(5):947-953.
APA	Li, Aifeng,Tian, Zhijia,Li, Jing,Yu, Rencheng,Banack, Sandra Anne,&Wang, Zhenyu.(2010).Detection of the neurotoxin BMAA within cyanobacteria isolated from freshwater in China.TOXICON,55(5),947-953.
MLA	Li, Aifeng,et al."Detection of the neurotoxin BMAA within cyanobacteria isolated from freshwater in China".TOXICON 55.5(2010):947-953.

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