Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta)

doi:10.1007/s11105-009-0170-8

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	Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta)
	Fu, Wandong 1,2; Li Shuai 3; Yao, Jianting 1; Yu, Shenhui 1; Liu, Fuli 1; Duan, Delin 1
	2010-09-01
发表期刊	PLANT MOLECULAR BIOLOGY REPORTER
卷号	28 期号:3 页码:430-437
文章类型	Article
摘要	Heat shock protein 70 (Hsp70) is one of the important members of heat shock protein (Hsp) families and plays essential roles in folding nascent protein, translocation, refolding denatured protein, protein degradation, adverse stress resistance, and so on. In this study, homologous cloning coupled with the rapid amplification of cDNA ends was used to clone full-length cytosolic heat shock protein 70 of Enteromorpha prolifera (designed as EPHsp70). Bioinformatics was used to analyze structural feature, homologous relationship, and phylogenetic position of EPHsp70. The full length of EPHsp70 cDNA was 2,265 bp, with a 5' untranslated region of 65 bp, a 3' untranslated region of 217 bp, and an open-reading frame of 1,983 bp encoding a polypeptide of 660 amino acids with an estimated molecular weight of 71.39 kDa and an estimated isoelectric point of 5.03. EPHsp70 had five degenerate repeats of tetrapeptide GGMP and three typical Hsp70 signature motifs. The C-terminus amino acid sequence of cytosolic EPHsp70 was EEVD, and the conservation of EPHsp70 of N-terminus was higher than that of C-terminus. The homology between EPHsp70 and the cytosolic Hsp70s of other algae and land plants was more than 70%.
关键词	Enteromorpha Prolifera Heat Shock Protein 70 Molecular Cloning
DOI	10.1007/s11105-009-0170-8
收录类别	SCI
语种	英语
WOS记录号	WOS:000276440500009
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32687
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Zhejiang Marine Dev Res Inst, Zhoushan 316100, Peoples R China 3.Qingdao Univ, Coll Chem Chem Engn & Environm Sci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Fu, Wandong,Li Shuai,Yao, Jianting,et al. Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta)[J]. PLANT MOLECULAR BIOLOGY REPORTER,2010,28(3):430-437.
APA	Fu, Wandong,Li Shuai,Yao, Jianting,Yu, Shenhui,Liu, Fuli,&Duan, Delin.(2010).Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta).PLANT MOLECULAR BIOLOGY REPORTER,28(3),430-437.
MLA	Fu, Wandong,et al."Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta)".PLANT MOLECULAR BIOLOGY REPORTER 28.3(2010):430-437.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
Molecular Cloning an（770KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览