Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
| Determination of sulphate-reducing bacteria based on vancomycin-functionalised magnetic nanoparticles using a modification-free quartz crystal microbalance | |
| Wan, Yi1,2; Zhang, Dun1; Hou, Baorong1 | |
| 2010-03-15 | |
| 发表期刊 | BIOSENSORS & BIOELECTRONICS
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| 卷号 | 25期号:7页码:1847-1850 |
| 文章类型 | Article |
| 摘要 | A fast, sensitive and reliable quartz crystal microbalance (QCM) biosensor is described for the selective detection of the marine pathogenic sulphate-reducing bacterium (SRB), D. desulfotomaculum. Based on the amplification of the response of vancomycin-functionalised magnetic nanoparticles (Van-mNPs), under an external magnetic field, the bacteria-mNPs conjugates attach to the surface of an Au electrode. The QCM biosensor gave a distinct response to the vancomycin-sensitive, D. desulfotomaculum, but had no obvious response to the vancomycin-resistant bacterium, Vibrio anguillarum. The effects of the optimization conditions such as the incubation time and pH on the detection were also investigated, respectively. optimised assays showed that the biosensor could obtain the best response with a 30 min incubation of the bacteria with the Van-mNPs. A linear relationship between the QCM response and the logarithm of the bacterial concentration was observed in the range of 1.8 x 10(4) to 1.8 x 10(7) cfu/ml. The sensor system has a potential for further applications and provides a facile and sample method for detection of pathogenic bacteria. (C) 2009 Elsevier B.V. All rights reserved. |
| 关键词 | Sulphate-reducing Bacteria Vancomycin Magnetic Nanoparticle Quartz Crystal Microbalance |
| DOI | 10.1016/j.bios.2009.12.028 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275978700049 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32672 |
| 专题 | 海洋环境腐蚀与生物污损重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Yi,Zhang, Dun,Hou, Baorong. Determination of sulphate-reducing bacteria based on vancomycin-functionalised magnetic nanoparticles using a modification-free quartz crystal microbalance[J]. BIOSENSORS & BIOELECTRONICS,2010,25(7):1847-1850. |
| APA | Wan, Yi,Zhang, Dun,&Hou, Baorong.(2010).Determination of sulphate-reducing bacteria based on vancomycin-functionalised magnetic nanoparticles using a modification-free quartz crystal microbalance.BIOSENSORS & BIOELECTRONICS,25(7),1847-1850. |
| MLA | Wan, Yi,et al."Determination of sulphate-reducing bacteria based on vancomycin-functionalised magnetic nanoparticles using a modification-free quartz crystal microbalance".BIOSENSORS & BIOELECTRONICS 25.7(2010):1847-1850. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Determination of sul（328KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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