Metallothionein and superoxide dismutase responses to sublethal cadmium exposure in the clam Mactra veneriformis

doi:10.1016/j.cbpc.2009.12.005

	Metallothionein and superoxide dismutase responses to sublethal cadmium exposure in the clam Mactra veneriformis
	Fang, Yan 2; Yang, Hongsheng1 ; Wang, Tianming 2; Liu, Baozhong; Zhao, Heling 2; Chen, Muyan
	2010-04-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
卷号	151 期号:3 页码:325-333
文章类型	Article
摘要	The objective of this study was to identify the relationship between cadmium (Cd) and stress responses in the clam Mactra veneriformis. Metallothionein (MT) and Cu-Zn superoxide dismutase (SOD) cDNAs from the clam were isolated and characterized. The full-length cDNA of MvMT and MvSOD contained 830 and 689 nucleotides encoding 59 and 159 amino acids, respectively. Multiple alignments indicated that deduced amino acid sequences of MvMT and MvSOD shared high homology with MT and SOD sequences of other mollusks. Clams were exposed to 0, 50, 100, and 200 mu g/L Cd for 21 days. The mRNA transcripts of the two genes, MT protein content and SOD activity in the digestive gland were examined. Cd treatment significantly elevated MvMT and MvSOD mRNA expression in a dose-dependent manner and MT protein level in a dose-and time-dependent manner. SOD activity significantly increased at the start of CA exposure, then decreased and finally returned to the normal level. These results indicate that MT and SOD play an important role in maintaining cellular metabolism homeostasis and protecting M. veneriformis from Cd toxicity. MT and SOD could be used as biomarkers; of CA pollution in aquatic environment for the studied species. (C) 2009 Elsevier Inc. All rights reserved.
关键词	Cadmium Mactra Veneriformis Metallothionein Mrna Expression Superoxide Dismutase
DOI	10.1016/j.cbpc.2009.12.005
收录类别	SCI
语种	英语
WOS记录号	WOS:000275627400009
引用统计	被引频次：75[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32666
专题	海洋生态与环境科学重点实验室实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fang, Yan,Yang, Hongsheng,Wang, Tianming,et al. Metallothionein and superoxide dismutase responses to sublethal cadmium exposure in the clam Mactra veneriformis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2010,151(3):325-333.
APA	Fang, Yan,Yang, Hongsheng,Wang, Tianming,Liu, Baozhong,Zhao, Heling,&Chen, Muyan.(2010).Metallothionein and superoxide dismutase responses to sublethal cadmium exposure in the clam Mactra veneriformis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,151(3),325-333.
MLA	Fang, Yan,et al."Metallothionein and superoxide dismutase responses to sublethal cadmium exposure in the clam Mactra veneriformis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 151.3(2010):325-333.

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