Cloning and characterization of NM23-Bbt2 gene from amphioxus Branchiostoma belcheri tsingtauense

doi:10.2478/s11756-009-0124-5

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	Cloning and characterization of NM23-Bbt2 gene from amphioxus Branchiostoma belcheri tsingtauense
	Xing, Fuguo 1; Tan, Xungang2 ; Zhang, Pei-Jun 2; Liu, Yang 1; Ma, Junkai 2
	2009-08-01
发表期刊	BIOLOGIA
卷号	64 期号:4 页码:803-810
文章类型	Article
摘要	A full-length amphioxus (Branchiostoma belcheri tsingtauense) NM23-Bbt2, NM23-H2 homologue, cDNA was isolated from the cDNA library and sequenced. The obtained amphioxus NM23-Bbt2 cDNA contains an open reading frame coding for 171 amino acids. Sequence analysis showed that the amphioxus NM23-Bbt2 was highly conserved with that of other species, and all of them contained highly conserved motifs that play important roles in the function of NM23. RT-PCR revealed that NM23-Bbt2 is expressed in the neuronal tissues and is expressed in all stages during the embryogenesis. Nucleoside kinases are thought to have a critical role in regulatory processes such as signal transduction, proliferation, and differentiation. Taken together, these results suggest that nucleoside diphosphate kinases have an important role to play in embryogenic development in amphioxus. Phylogenetic analysis showed that the amphioxus group 1 NDPKs (Bf1-4) may be precursors of the human group 1 NDPKs, NM23-Bf5, NM23-Bf6, NM23-Bf7 and NM23-Bf8 may be precursors of NM23-H5, NM23-H6, NM23-H7 and M23-H8, respectively. Our finding of nine NM23 genes in Branchiostoma floride, the precursor of vertebrates, strongly suggests that the ancestral gene corresponding to each of vertebrates NM23 genes generated before the appearance of vertebrates. Comparison of the gene structures of NM23-H2 homologue from invertebrates to vertebrates suggests that the locations of three of the four introns are conserved in amphioxus and vertebrates.
关键词	Amphioxus Cephalochordate Evolution Expression Ndpk Nm23-bbt2
DOI	10.2478/s11756-009-0124-5
收录类别	SCI
语种	英语
WOS记录号	WOS:000268190600028
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32651
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Agr Sci, Inst Agro Food Sci & Technol, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Xing, Fuguo,Tan, Xungang,Zhang, Pei-Jun,et al. Cloning and characterization of NM23-Bbt2 gene from amphioxus Branchiostoma belcheri tsingtauense[J]. BIOLOGIA,2009,64(4):803-810.
APA	Xing, Fuguo,Tan, Xungang,Zhang, Pei-Jun,Liu, Yang,&Ma, Junkai.(2009).Cloning and characterization of NM23-Bbt2 gene from amphioxus Branchiostoma belcheri tsingtauense.BIOLOGIA,64(4),803-810.
MLA	Xing, Fuguo,et al."Cloning and characterization of NM23-Bbt2 gene from amphioxus Branchiostoma belcheri tsingtauense".BIOLOGIA 64.4(2009):803-810.

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