Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-phycocyanin in Escherichia coli by using one expression vector | |
| Guan, Xiangyu; Qin, Song; Su, Zhongliang; Zhao, Fangqing; Ge, Baosheng; Li, Fuchao; Tang, Xuexi | |
| 2007-07-01 | |
| 发表期刊 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 卷号 | 142期号:1页码:52-59 |
| 文章类型 | Article |
| 摘要 | The phycobiliproteins (PBSs) are large pigment proteins found in certain algae that play a central role in harvesting light energy for photosynthesis. Phycocyanin ( PC) is one type of PBSs that gains increasing attention owing to its various biological and pharmacological properties. In this paper, an expression vector containing five essential genes in charge of biosynthesis of cyanobacterial C-phycocyanin (C-PC) holo-alpha subunit (holo-CpcA) was successfully constructed resulting in over-expression of a fluorescent holo-CpcA in E. coli BL21. The vector harbored two cassettes: one cassette carried genes hox1 and pcyA required for conversion of heme to phycocyanobilin (PCB), and the other cassette carried cpcA encoding CpcA along with cpcE and cpcF both of which were necessary and sufficient for the correct addition of PCB to CpcA. The vector system contained a His-tag for protein purification. The purified protein showed correct molecular weight on SDS-PAGE gel and emitted orange fluorescence by UV excitation. The maximum peak of absorbance spectrum was at 623 nm, and the maximum peak of fluorescence emission and excitation were at 648 and 633 nm, respectively, which were similar to those of native C-PC. This study provides an efficient method for large-scale production of the fluorescent holo-CpcA in biotechnological applications. |
| 关键词 | Escherichia Coli Phycocyanin Fluorescence Holo-ht-cpca |
| DOI | 10.1007/s12010-007-8000-7 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000250069800005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32589 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Fac Life Sci & Technol, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Univ Qingdao Sci & Technol, Qingdao 266006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guan, Xiangyu,Qin, Song,Su, Zhongliang,et al. Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-phycocyanin in Escherichia coli by using one expression vector[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2007,142(1):52-59. |
| APA | Guan, Xiangyu.,Qin, Song.,Su, Zhongliang.,Zhao, Fangqing.,Ge, Baosheng.,...&Tang, Xuexi.(2007).Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-phycocyanin in Escherichia coli by using one expression vector.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,142(1),52-59. |
| MLA | Guan, Xiangyu,et al."Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-phycocyanin in Escherichia coli by using one expression vector".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 142.1(2007):52-59. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Combinational biosyn（176KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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