Cloning, expression and characterization of phycoerythrin gene from Ceramium boydenn

doi:10.1080/10425170600724907

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	Cloning, expression and characterization of phycoerythrin gene from Ceramium boydenn
	Zhang, Xiaowen ; Zhao, Fangqing ; Qin, Song ; Yan, Binlun
	2006-04-01
发表期刊	DNA SEQUENCE
卷号	17 期号:2 页码:129-135
文章类型	Article
摘要	Phycobiliproteins function as a major light harvesting protein-pigment complex in the cyanobacteria and the eukaryotic algae. Phycoerythrin (PE) is a kind of phycobiliproteins, widely located in all rhodophytes, some species of cyanobacteria and cryptophytes, and different ecotypes of Prochlorococcus populations. PeBA encoding beta and alpha subunits of PE from Ceramium boydenn was cloned and sequenced in this research. A peBA specific PCR primer was synthesized, based on the peBA gene conserved sequences. The a subunit encoding gene (peB) contained an open reading frame of 534 bp, while the a subunit (peA) was 495 bp. Recombinant expression plasmid pET-peAB was constructed and expressed in Escherichia coli BL21. The molecular weight of expressive product of peB and peA was about 23.3 and 18.2 KD, respectively. Results of codon usage analysis show that G + C content is heterogeneous among different groups of PE and spacers have dramatically lower G + C contents than coding regions. Also there is a high variance in G + C content among sequences at the third position sites. It is also found in this paper that several sequence regions, which might reflect functional or structural requirements of the PE organization, and several residues known for their functional importance are conserved in almost all the sequences.
关键词	Phycoerythrin Clone Expression Codon Usage Alignment Phycobiliproteins Ceramium Boydenn Rhodophytes
DOI	10.1080/10425170600724907
收录类别	SCI
语种	英语
WOS记录号	WOS:000240356000007
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32553
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Huaihai Inst Technol, Lianyungang, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaowen,Zhao, Fangqing,Qin, Song,et al. Cloning, expression and characterization of phycoerythrin gene from Ceramium boydenn[J]. DNA SEQUENCE,2006,17(2):129-135.
APA	Zhang, Xiaowen,Zhao, Fangqing,Qin, Song,&Yan, Binlun.(2006).Cloning, expression and characterization of phycoerythrin gene from Ceramium boydenn.DNA SEQUENCE,17(2),129-135.
MLA	Zhang, Xiaowen,et al."Cloning, expression and characterization of phycoerythrin gene from Ceramium boydenn".DNA SEQUENCE 17.2(2006):129-135.

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