Optimization of triploid induction by heat shock in Chinese shrimp Fenneropenaeus chinensis

doi:10.1016/S0044-8486(03)00006-1

IOCAS-IR > 实验海洋生物学重点实验室

	Optimization of triploid induction by heat shock in Chinese shrimp Fenneropenaeus chinensis
	Li, FH; Xiang, JH; Zhou, LH; Wu, CG; Zhang, XJ
	2003-04-02
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	219 期号:1-4 页码:221-231
文章类型	Article
摘要	Chromosome manipulation for commercially valuable marine animals plays an important role in aquaculture. The special reproductive characteristics of shrimp make it difficult to control fertilization and synchronize egg development, so research on chromosome manipulation in shrimp has proceeded very slowly. In the present study, triploid shrimp Fenneropenaeus chinensis were induced by heat shocks and the optimal-inducing condition was screened at different spawning temperatures. Level of triploid induction for each treatment was evaluated by flow cytometry at nauplius stage. The highest level of triploid induction reached to more than 90%. Starting time for each treatment was very crucial for triploid induction in shrimp. One optimal treatment condition for triploid induction was heat shock (29-32 degreesC), starting at 18-20 min for duration of 10 min. These conditions varied depending on the temperature at spawning. Triploid level at embryo stage and nauplius stage was not different, suggesting the same hatching rate between diploids and triploids. Heat shock is a very effective way to induce triploids in this species, and can be easily used on large scale without any harmful effect on the environment as compared with chemical treatment. (C) 2003 Elsevier Science B.V. All rights reserved.; Chromosome manipulation for commercially valuable marine animals plays an important role in aquaculture. The special reproductive characteristics of shrimp make it difficult to control fertilization and synchronize egg development, so research on chromosome manipulation in shrimp has proceeded very slowly. In the present study, triploid shrimp Fenneropenaeus chinensis were induced by heat shocks and the optimal-inducing condition was screened at different spawning temperatures. Level of triploid induction for each treatment was evaluated by flow cytometry at nauplius stage. The highest level of triploid induction reached to more than 90%. Starting time for each treatment was very crucial for triploid induction in shrimp. One optimal treatment condition for triploid induction was heat shock (29-32 degreesC), starting at 18-20 min for duration of 10 min. These conditions varied depending on the temperature at spawning. Triploid level at embryo stage and nauplius stage was not different, suggesting the same hatching rate between diploids and triploids. Heat shock is a very effective way to induce triploids in this species, and can be easily used on large scale without any harmful effect on the environment as compared with chemical treatment. (C) 2003 Elsevier Science B.V. All rights reserved.
关键词	Triploid Heat Shock Chinese Shrimp Fenneropenaeus Chinensis
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1016/S0044-8486(03)00006-1
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000181802700017
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3254
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, FH,Xiang, JH,Zhou, LH,et al. Optimization of triploid induction by heat shock in Chinese shrimp Fenneropenaeus chinensis[J]. AQUACULTURE,2003,219(1-4):221-231.
APA	Li, FH,Xiang, JH,Zhou, LH,Wu, CG,&Zhang, XJ.(2003).Optimization of triploid induction by heat shock in Chinese shrimp Fenneropenaeus chinensis.AQUACULTURE,219(1-4),221-231.
MLA	Li, FH,et al."Optimization of triploid induction by heat shock in Chinese shrimp Fenneropenaeus chinensis".AQUACULTURE 219.1-4(2003):221-231.

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