An improved method for isolation of photosystem II from marine alga Porphyra yezoensis Udea

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	An improved method for isolation of photosystem II from marine alga Porphyra yezoensis Udea
	Gao, ZQ ; Wang, GC ; Tseng, CK
	2005-02-01
发表期刊	INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
卷号	42 期号:1 页码:41-47
文章类型	Article
摘要	An improved method for isolation and characterization of photosystem (PS)II particles from thylakoid membranes of gametophytes. of a marine alga Porphyra yezoensis Udea is reported. Thylakoid membranes were isolated using ultracentrifugation and differential speeds centrifugation and were further purified by the first sucrose density gradient centrifugation (SDGC). PSII particles with high 2, 6-dichloroindophenol (DCIP) photo-reduction activity were isolated by the second SDGC from the thylakoid membranes. Absorption and fluorescence spectra of the thylakoid membranes and PSII particles were recorded and their polypeptides composition was studied. Thylakoid membranes obtained by the above two methods showed similar spectral properties and polypeptides composition. PSII particles, in addition to common extrinsic proteins found in PSII of other plants, contained cyt c-550, a 20 kDa protein, along with two new proteins (14 kDa and 16 kDa).
关键词	Porphyra Yezoensis Udea Thylakoid Membranes Psii Ultracentrifugation Differential Speeds Centrifugation Sds-page Absorption Spectra Fluorescence Spectra
收录类别	SCI
语种	英语
WOS记录号	WOS:000227505100006
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32525
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Gao, ZQ,Wang, GC,Tseng, CK. An improved method for isolation of photosystem II from marine alga Porphyra yezoensis Udea[J]. INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,2005,42(1):41-47.
APA	Gao, ZQ,Wang, GC,&Tseng, CK.(2005).An improved method for isolation of photosystem II from marine alga Porphyra yezoensis Udea.INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,42(1),41-47.
MLA	Gao, ZQ,et al."An improved method for isolation of photosystem II from marine alga Porphyra yezoensis Udea".INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS 42.1(2005):41-47.

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