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| Characterization and expression profile of AmphiCD63 encoding a novel member of TM4SF proteins from amphioxus Branchiostoma belcheri tsingtauense | |
Liu, ZH ; Zhang, SC; Li, HY; Luan, J; Wang, YJ; Wang, L; Xiang, JH
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| 2005-06-01 | |
| 发表期刊 | DNA SEQUENCE
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| 卷号 | 16期号:3页码:195-201 |
| 文章类型 | Article |
| 摘要 | The study on CD antigen genes remains lacking in the cephalochordate amphioxus to date. In this report, the cDNA encoding CD63 was identified for the first time from the gut cDNA library of amphioxus Branchiostoma belcheri tsingtauense. Primary structural examination showed that the protein encoded by the cDNA contained four potential transmembrane domains characteristic of transmembrane 4 superfamily ( TM4SF) proteins and a conserved CCG motif in the putative major extracellular loop. BLAST search revealed that the cDNA is closely associated with other known CD63 antigen genes, and it was thus designated AmphiCD63. Phylogenetic analysis indicated that AmphiCD63 is extremely close to vertebrate CD63, CD151 and CD53, suggesting they may have been evolved from a common ancestral gene. RT-PCR analysis exhibited that AmphiCD63 mRNA was abundant in muscle, ovary, foregut including hepatic caecum and hindgut, while it was present at considerably lower levels in notochord and gill and absent in testis. |
| 关键词 | Amphioxus Branchiostoma Cd63 Evolution Expression Tm4sf |
| DOI | 10.1080/10425170500097883 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230506200006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32512 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Dept Marine Biol, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, ZH,Zhang, SC,Li, HY,et al. Characterization and expression profile of AmphiCD63 encoding a novel member of TM4SF proteins from amphioxus Branchiostoma belcheri tsingtauense[J]. DNA SEQUENCE,2005,16(3):195-201. |
| APA | Liu, ZH.,Zhang, SC.,Li, HY.,Luan, J.,Wang, YJ.,...&Xiang, JH.(2005).Characterization and expression profile of AmphiCD63 encoding a novel member of TM4SF proteins from amphioxus Branchiostoma belcheri tsingtauense.DNA SEQUENCE,16(3),195-201. |
| MLA | Liu, ZH,et al."Characterization and expression profile of AmphiCD63 encoding a novel member of TM4SF proteins from amphioxus Branchiostoma belcheri tsingtauense".DNA SEQUENCE 16.3(2005):195-201. |
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