Cloning and characterization of the phycoerythrin operon upstream sequence of Gracilaria lemaneiformis (Rhodophyta)

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	Cloning and characterization of the phycoerythrin operon upstream sequence of Gracilaria lemaneiformis (Rhodophyta)
	Sui, ZH ; Zhang, XC ; Ren, XY; Liu, JJ ; Mao, YX ; Qin, S
	2004-06-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
卷号	16 期号:3 页码:167-174
文章类型	Article
摘要	A study was made of the regulatory region of the gene responsible for the expression of phycoerythrin in the red alga Gracilaria lemaneiformis. Rapid Amplified cDNA Ends Polymerase Chain Reaction (RACE-PCR) was used to obtain the 5' untranslated sequence of phycoerythrin operon of the alga. The transcriptional initiation site starting with A was found at 80 bp ahead of the AUG codon of the structural gene. A 54 bp fragment with two or three gaps inside was determined to be 66.7% similar to 85.2% similar to those of other red algae. In vitro PCR cloning technique was used to clone the far upstream untranscribed sequence. There was no significant similarity of untranscribed sequence to those known for other red algae. Neither a canonical TATA nor CAAT box was found in this region. As predicted by PLACE, a software suitable for plant promoter analysis, there were two special functional area, one around nucleotide 65, the other 328, responsible for light regulated and / or high level of gene expression. The untranscribed region of phycoerythrin operon was subcloned into a promoter report vector, pEGFP, and transferred into E. coli and Synechocystis PCC6803, respectively. Strong green fluorescence was observed in electroporated Synechocystis PCC 6803 cells 24hrs after the operating, but not in E. coli, suggesting that the promoter is transactivated.
关键词	Gracilaria Lemaneiformis In Vitro Pcr clonIng Phycoerythrin Promoter Race-pcr Transcriptional Initiation Site
收录类别	SCI
语种	英语
WOS记录号	WOS:000225180200002
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32501
专题	实验海洋生物学重点实验室
作者单位	1.Ocean Univ Qingdao, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Beijing 100864, Peoples R China
推荐引用方式 GB/T 7714	Sui, ZH,Zhang, XC,Ren, XY,et al. Cloning and characterization of the phycoerythrin operon upstream sequence of Gracilaria lemaneiformis (Rhodophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2004,16(3):167-174.
APA	Sui, ZH,Zhang, XC,Ren, XY,Liu, JJ,Mao, YX,&Qin, S.(2004).Cloning and characterization of the phycoerythrin operon upstream sequence of Gracilaria lemaneiformis (Rhodophyta).JOURNAL OF APPLIED PHYCOLOGY,16(3),167-174.
MLA	Sui, ZH,et al."Cloning and characterization of the phycoerythrin operon upstream sequence of Gracilaria lemaneiformis (Rhodophyta)".JOURNAL OF APPLIED PHYCOLOGY 16.3(2004):167-174.

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