Genomic cloning, expression and recombinant protein purification of alpha and beta subunits of the allophycocyanin gene (apc) from the cyanobacterium Anacystis nidulans UTEX 625

doi:10.1007/s10811-005-5561-0

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	Genomic cloning, expression and recombinant protein purification of alpha and beta subunits of the allophycocyanin gene (apc) from the cyanobacterium Anacystis nidulans UTEX 625
	Qin, S ; Tang, ZH ; Lin, F ; Sung, LPA ; Tseng, CK
	2004-12-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	16 期号:6 页码:483-487
文章类型	Article
摘要	A genomic fragment encoding alpha(APC) and beta(APC) (i.e., alpha and beta units of the allophycocyanin, APC) from Anacystis nidulans UTEX 625 was cloned and sequenced. This fragment, containing a non-coding sequence of 56 nucleotides in between, was then subcloned into the expression vector pMal-c2 downstream from and in frame with the malE gene of E. coli encoding MBP ( maltose binding protein). The fusion protein was purified by amylose affinity chromatography and cleaved by coagulation factor Xa. alpha(APC) and beta(APC) were then separated from MBP and MBP fusion proteins, respectively, and concentrated by membrane centrifugation. The study provides a method to produce recombinant allophycocyanin subunits for biomedical and biotechnological applications.; A genomic fragment encoding alpha(APC) and beta(APC) (i.e., alpha and beta units of the allophycocyanin, APC) from Anacystis nidulans UTEX 625 was cloned and sequenced. This fragment, containing a non-coding sequence of 56 nucleotides in between, was then subcloned into the expression vector pMal-c2 downstream from and in frame with the malE gene of E. coli encoding MBP ( maltose binding protein). The fusion protein was purified by amylose affinity chromatography and cleaved by coagulation factor Xa. alpha(APC) and beta(APC) were then separated from MBP and MBP fusion proteins, respectively, and concentrated by membrane centrifugation. The study provides a method to produce recombinant allophycocyanin subunits for biomedical and biotechnological applications.
关键词	Allophycocyanin Gene Anacystis Nidulans Cyanobacterium Synechococcus Pcc 6301 Recombinant Protein
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10811-005-5561-0
URL	查看原文
收录类别	ISTP ; SCI
语种	英语
WOS记录号	WOS:000230724000010
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3212
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
推荐引用方式 GB/T 7714	Qin, S,Tang, ZH,Lin, F,et al. Genomic cloning, expression and recombinant protein purification of alpha and beta subunits of the allophycocyanin gene (apc) from the cyanobacterium Anacystis nidulans UTEX 625[J]. JOURNAL OF APPLIED PHYCOLOGY,2004,16(6):483-487.
APA	Qin, S,Tang, ZH,Lin, F,Sung, LPA,&Tseng, CK.(2004).Genomic cloning, expression and recombinant protein purification of alpha and beta subunits of the allophycocyanin gene (apc) from the cyanobacterium Anacystis nidulans UTEX 625.JOURNAL OF APPLIED PHYCOLOGY,16(6),483-487.
MLA	Qin, S,et al."Genomic cloning, expression and recombinant protein purification of alpha and beta subunits of the allophycocyanin gene (apc) from the cyanobacterium Anacystis nidulans UTEX 625".JOURNAL OF APPLIED PHYCOLOGY 16.6(2004):483-487.

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