An efficient method for DNA isolation from red algae

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	An efficient method for DNA isolation from red algae
	Hu, ZN ; Zeng, XQ ; Wang, AH ; Shi, CJ ; Duan, DL
	2004-06-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	16 期号:3 页码:161-166
文章类型	Article
摘要	A simple, inexpensive and efficient method was developed for rapid isolation of total genomic DNA from 15 red algal species. It resulted in 0.1 mug high quality DNA from 1 mg fresh algal material, with an A(260)/A(280) ratio of 1.68 - 1.90. Using this rapidly isolated DNA, the 18S ribosomal RNA genes ( rDNA) and the nuclear ribosomal DNA of the internal transcribed spacer (ITS) regions were amplified. The tested DNA was suitable for restriction endonuclease digestion, genetic marker analysis and polymerase chain reaction (PCR) amplification, and may be valid for other genetic manipulation.; A simple, inexpensive and efficient method was developed for rapid isolation of total genomic DNA from 15 red algal species. It resulted in 0.1 mug high quality DNA from 1 mg fresh algal material, with an A(260)/A(280) ratio of 1.68 - 1.90. Using this rapidly isolated DNA, the 18S ribosomal RNA genes ( rDNA) and the nuclear ribosomal DNA of the internal transcribed spacer (ITS) regions were amplified. The tested DNA was suitable for restriction endonuclease digestion, genetic marker analysis and polymerase chain reaction (PCR) amplification, and may be valid for other genetic manipulation.
关键词	Dna Isolation Genetic Manipulation Pcr Amplification Polysaccharides Red Algae
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000225180200001
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3196
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ Qingdao, Coll Fisheries, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Hu, ZN,Zeng, XQ,Wang, AH,et al. An efficient method for DNA isolation from red algae[J]. JOURNAL OF APPLIED PHYCOLOGY,2004,16(3):161-166.
APA	Hu, ZN,Zeng, XQ,Wang, AH,Shi, CJ,&Duan, DL.(2004).An efficient method for DNA isolation from red algae.JOURNAL OF APPLIED PHYCOLOGY,16(3),161-166.
MLA	Hu, ZN,et al."An efficient method for DNA isolation from red algae".JOURNAL OF APPLIED PHYCOLOGY 16.3(2004):161-166.

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