Characterization of the main light-harvesting chlorophyll a/b-protein complex of green alga, Bryopsis corticulans

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	Characterization of the main light-harvesting chlorophyll a/b-protein complex of green alga, Bryopsis corticulans
	Chen, H; Shen, SH ; Gong, YD ; He, JF ; Wang, GC ; Li, LB ; Kuang, TY
	2004-10-01
发表期刊	ACTA BOTANICA SINICA
ISSN	1672-6650
卷号	46 期号:10 页码:1192-1199
文章类型	Article
摘要	The main light-harvesting chlorophyll a/b -protein complex (LHC II) has been isolated directly from thylakoid membranes of shiphonous green alga, Bryopsis corticulans Setch. by using two consecutive runs of anion exchange and gel-filtration chromatography. Monomeric and trimeric subcomplexes of LHC 11 were obtained by using sucrose gradient ultracentrifugation. Pigment analysis by reversed-phase high performance liquid chromatography showed that chlorophyll a (Chl a), chlorophyll b (Chl b), neoxanthin, violaxanthin and siphonaxanthin were involved in LHC 11 from B. corticulans. The properties of electronic transition of monomeric LHC II showed similarities to those of trimeric LHC II. Circular dichroism spectroscopy showed that strong intramolecular interaction of excitonic dipoles between Chl a and between Chl b exist in one LHC II apoprotein, while the intermolecular interaction of these dipoles can be intensified in the trimeric structure. The monomer has high efficient energy transfer from Chl b and siphonaxanthin to Chl a similarly to that of the trimer. Our results suggest that in B. corticulans, LHC II monomer has high ordered pigment organization that play effective physiological function as the trimer, and thus it might be also a functional organization existing in thylakoid membrane of B. corticulans.; The main light-harvesting chlorophyll a/b -protein complex (LHC II) has been isolated directly from thylakoid membranes of shiphonous green alga, Bryopsis corticulans Setch. by using two consecutive runs of anion exchange and gel-filtration chromatography. Monomeric and trimeric subcomplexes of LHC 11 were obtained by using sucrose gradient ultracentrifugation. Pigment analysis by reversed-phase high performance liquid chromatography showed that chlorophyll a (Chl a), chlorophyll b (Chl b), neoxanthin, violaxanthin and siphonaxanthin were involved in LHC 11 from B. corticulans. The properties of electronic transition of monomeric LHC II showed similarities to those of trimeric LHC II. Circular dichroism spectroscopy showed that strong intramolecular interaction of excitonic dipoles between Chl a and between Chl b exist in one LHC II apoprotein, while the intermolecular interaction of these dipoles can be intensified in the trimeric structure. The monomer has high efficient energy transfer from Chl b and siphonaxanthin to Chl a similarly to that of the trimer. Our results suggest that in B. corticulans, LHC II monomer has high ordered pigment organization that play effective physiological function as the trimer, and thus it might be also a functional organization existing in thylakoid membrane of B. corticulans.
关键词	Light-harvesting Protein Complex Monomer Pigment Composition Pigment Interaction Bryopsis Corticulans
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000224622100009
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3180
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Technol, Xian 710068, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Chen, H,Shen, SH,Gong, YD,et al. Characterization of the main light-harvesting chlorophyll a/b-protein complex of green alga, Bryopsis corticulans[J]. ACTA BOTANICA SINICA,2004,46(10):1192-1199.
APA	Chen, H.,Shen, SH.,Gong, YD.,He, JF.,Wang, GC.,...&Kuang, TY.(2004).Characterization of the main light-harvesting chlorophyll a/b-protein complex of green alga, Bryopsis corticulans.ACTA BOTANICA SINICA,46(10),1192-1199.
MLA	Chen, H,et al."Characterization of the main light-harvesting chlorophyll a/b-protein complex of green alga, Bryopsis corticulans".ACTA BOTANICA SINICA 46.10(2004):1192-1199.

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