A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms

doi:10.1080/13880200490885987

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	A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms
	Chen, HM ; Yan, XJ ; Lin, W ; Zheng, L ; Zhang, WW
	2004-09-01
发表期刊	PHARMACEUTICAL BIOLOGY
ISSN	1388-0209
卷号	42 期号:6 页码:416-421
文章类型	Article
摘要	A new enzyme assay method for screening alpha-glucosidase inhibitors with rapidity and simplicity was developed. The enzyme-substituted alpha-glucosidases for this assay was glucoamylase. Samples were spotted or developed on the silica gel plate. The agar solution containing substrate was poured on the plate, and paper impregnated with enzyme was layered on the agar. After incubation, an inhibitory circle would appear around the inhibitor. By using this method, more than 200 strains of marine microorganisms were screened. Among them, three active strains were found to secrete inhibitors in the culture medium.; A new enzyme assay method for screening alpha-glucosidase inhibitors with rapidity and simplicity was developed. The enzyme-substituted alpha-glucosidases for this assay was glucoamylase. Samples were spotted or developed on the silica gel plate. The agar solution containing substrate was poured on the plate, and paper impregnated with enzyme was layered on the agar. After incubation, an inhibitory circle would appear around the inhibitor. By using this method, more than 200 strains of marine microorganisms were screened. Among them, three active strains were found to secrete inhibitors in the culture medium.
关键词	Enzyme Assay Alpha-glucosidase Inhibitor Marine Microorganism
学科领域	Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
DOI	10.1080/13880200490885987
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000226444900006
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3178
专题	实验海洋生物学重点实验室
作者单位	1.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Chen, HM,Yan, XJ,Lin, W,et al. A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms[J]. PHARMACEUTICAL BIOLOGY,2004,42(6):416-421.
APA	Chen, HM,Yan, XJ,Lin, W,Zheng, L,&Zhang, WW.(2004).A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms.PHARMACEUTICAL BIOLOGY,42(6),416-421.
MLA	Chen, HM,et al."A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms".PHARMACEUTICAL BIOLOGY 42.6(2004):416-421.

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