Molecular cloning and expression of interleukin 1beta (IL-1 beta) from red seabream (Pagrus major)

IOCAS-IR > 实验海洋生物学重点实验室

	Molecular cloning and expression of interleukin 1beta (IL-1 beta) from red seabream (Pagrus major)
	Cai, ZH ; Song, LS ; Gao, CP ; Wu, LT ; Qiu, LH
	2004-05-01
发表期刊	PROGRESS IN NATURAL SCIENCE
ISSN	1002-0071
卷号	14 期号:5 页码:396-402
文章类型	Article
摘要	The interleukin 1beta (IL-1beta) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1beta sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1beta (RS IL-1beta). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84 bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1beta genes. The RS IL-1beta had the highest homology with piscine IL-1beta according to phylogenetic tree analysis. The transcript expression was detected in blood, brain, gill, heart, head kidney, kidney, liver, muscle and spleen in the pathogen challenged and healthy red seabream by RTPCR. Results showed that the RS IL-1beta mRNA was constitutively expressed in most of the tissues both in stimulated and un-stimulated fish, and the expression could be enhanced by pathogen challenging.; The interleukin 1beta (IL-1beta) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1beta sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1beta (RS IL-1beta). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84 bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1beta genes. The RS IL-1beta had the highest homology with piscine IL-1beta according to phylogenetic tree analysis. The transcript expression was detected in blood, brain, gill, heart, head kidney, kidney, liver, muscle and spleen in the pathogen challenged and healthy red seabream by RTPCR. Results showed that the RS IL-1beta mRNA was constitutively expressed in most of the tissues both in stimulated and un-stimulated fish, and the expression could be enhanced by pathogen challenging.
关键词	Interleukin-1 Beta Homology Cloning Mrna Expression Red Seabream
学科领域	Multidisciplinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000221600300003
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3176
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Agr Coll, Dept Fisheries Sci, Tianjin 300381, Peoples R China 3.Ocean Univ Qingdao, Coll Life Sci, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Cai, ZH,Song, LS,Gao, CP,et al. Molecular cloning and expression of interleukin 1beta (IL-1 beta) from red seabream (Pagrus major)[J]. PROGRESS IN NATURAL SCIENCE,2004,14(5):396-402.
APA	Cai, ZH,Song, LS,Gao, CP,Wu, LT,&Qiu, LH.(2004).Molecular cloning and expression of interleukin 1beta (IL-1 beta) from red seabream (Pagrus major).PROGRESS IN NATURAL SCIENCE,14(5),396-402.
MLA	Cai, ZH,et al."Molecular cloning and expression of interleukin 1beta (IL-1 beta) from red seabream (Pagrus major)".PROGRESS IN NATURAL SCIENCE 14.5(2004):396-402.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
cai-Molecular clonin（3318KB）			限制开放	--	浏览