Cortactin mediated morphogenic cell movements during zebrafish (Danio rerio) gastrulation

doi:10.1360/062004-111

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	Cortactin mediated morphogenic cell movements during zebrafish (Danio rerio) gastrulation
	Yu, D ; Zhang, PJ ; Zhan, X
	2005-12-01
发表期刊	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN	1006-9305
卷号	48 期号:6 页码:601-607
文章类型	Article
摘要	Cell migration is essential to direct embryonic cells to specific sites at which their developmental fates are ultimately determined. However, the mechanism by which cell motility is regulated in embryonic development is largely unknown. Cortactin, a filamentous actin binding protein, is an activator of Arp2/3 complex in the nucleation of actin cytoskeleton at the cell leading edge and acts directly on the machinery of cell motility. To determine whether cortactin and Arp2/3 mediated actin assembly plays a role in the morphogenic cell movements during the early development of zebrafish, we initiated a study of cortactin expression in zebrafish embryos at gastrulating stages when massive cell migrations occur. Western blot analysis using a cortactin specific monoclonal antibody demonstrated that cortactin protein is abundantly present in embryos at the most early developmental stages. Immunostaining of whole-mounted embryo showed that cortactin immunoreactivity was associated with the embryonic shield, predominantly at the dorsal side of the embryos during gastrulation. In addition, cortactin was detected in the convergent cells of the epiblast and hypoblast, and later in the central nervous system. Immunofluorescent staining with cortactin and Arp3 antibodies also revealed that cortactin and Arp2/3 complex colocalized at the periphery and many patches associated with the cell-to-cell junction in motile embryonic cells. Therefore, our data suggest that cortactin and Arp2/3 mediated actin polymerization is implicated in the cell movement during gastrulation and perhaps the development of the central neural system as well.; Cell migration is essential to direct embryonic cells to specific sites at which their developmental fates are ultimately determined. However, the mechanism by which cell motility is regulated in embryonic development is largely unknown. Cortactin, a filamentous actin binding protein, is an activator of Arp2/3 complex in the nucleation of actin cytoskeleton at the cell leading edge and acts directly on the machinery of cell motility. To determine whether cortactin and Arp2/3 mediated actin assembly plays a role in the morphogenic cell movements during the early development of zebrafish, we initiated a study of cortactin expression in zebrafish embryos at gastrulating stages when massive cell migrations occur. Western blot analysis using a cortactin specific monoclonal antibody demonstrated that cortactin protein is abundantly present in embryos at the most early developmental stages. Immunostaining of whole-mounted embryo showed that cortactin immunoreactivity was associated with the embryonic shield, predominantly at the dorsal side of the embryos during gastrulation. In addition, cortactin was detected in the convergent cells of the epiblast and hypoblast, and later in the central nervous system. Immunofluorescent staining with cortactin and Arp3 antibodies also revealed that cortactin and Arp2/3 complex colocalized at the periphery and many patches associated with the cell-to-cell junction in motile embryonic cells. Therefore, our data suggest that cortactin and Arp2/3 mediated actin polymerization is implicated in the cell movement during gastrulation and perhaps the development of the central neural system as well.
关键词	Cortactin Arp2/3 Complex Zebrafish Cell Movements Morphogenesis Gastrulation Neurulation
学科领域	Biology
DOI	10.1360/062004-111
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000235013600008
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3162
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Expt Marine Biol Lab, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Maryland, Sch Med, Dept Canc Ctr, Greenebaum Canc Ctr, Rockville, MD 20855 USA
推荐引用方式 GB/T 7714	Yu, D,Zhang, PJ,Zhan, X. Cortactin mediated morphogenic cell movements during zebrafish (Danio rerio) gastrulation[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2005,48(6):601-607.
APA	Yu, D,Zhang, PJ,&Zhan, X.(2005).Cortactin mediated morphogenic cell movements during zebrafish (Danio rerio) gastrulation.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,48(6),601-607.
MLA	Yu, D,et al."Cortactin mediated morphogenic cell movements during zebrafish (Danio rerio) gastrulation".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 48.6(2005):601-607.

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