Identification of peptides that bind with thymidylate synthase RNA using mRNA display technique

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	Identification of peptides that bind with thymidylate synthase RNA using mRNA display technique
	Yan, S ; Niu, RL ; Zhang, PJ ; Lin, XK
	2005-11-01
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN	1000-3282
卷号	32 期号:11 页码:1081-1087
文章类型	Article
摘要	Using in vitro selection method to isolate nucleic acids, peptides and proteins has been studied intensively in recent years. In vitro mRNA display is a new and effective technique for peptides selection, and the rationale of this technique is that a synthetic mRNA with puromycin could covalently link with the protein that it encodes, thus an mRNA-protein fusion is formed. This approach has been used in identification of many functional peptides. The peptides binding with thymidylate synthase RNA were isolated using mRNA display technique from a large peptide library (>10(13) different sequences). The selection scheme was constructed, and the experimental conditions, including library synthesis, formation of RNA-peptide fusion and RNA immobilization were optimized. Eight cycles have been processed and the results confirmed that the selected peptides could bind with thymidylate synthase mRNA specifically. Compared the amino acid sequences of the selected peptides with those from the initial random library, the basic and aromatic residues in selected peptides were enriched significantly, suggesting these peptide regions may be important in the peptide-TS mRNA interaction. As a novel in vitro selection approach, mRNA display technique would be developed as a powerful tool for isolation of functional peptides and proteins that could interact with immobilized targets with high affinity and specificity.; Using in vitro selection method to isolate nucleic acids, peptides and proteins has been studied intensively in recent years. In vitro mRNA display is a new and effective technique for peptides selection, and the rationale of this technique is that a synthetic mRNA with puromycin could covalently link with the protein that it encodes, thus an mRNA-protein fusion is formed. This approach has been used in identification of many functional peptides. The peptides binding with thymidylate synthase RNA were isolated using mRNA display technique from a large peptide library (>10(13) different sequences). The selection scheme was constructed, and the experimental conditions, including library synthesis, formation of RNA-peptide fusion and RNA immobilization were optimized. Eight cycles have been processed and the results confirmed that the selected peptides could bind with thymidylate synthase mRNA specifically. Compared the amino acid sequences of the selected peptides with those from the initial random library, the basic and aromatic residues in selected peptides were enriched significantly, suggesting these peptide regions may be important in the peptide-TS mRNA interaction. As a novel in vitro selection approach, mRNA display technique would be developed as a powerful tool for isolation of functional peptides and proteins that could interact with immobilized targets with high affinity and specificity.
关键词	In Vitro Mrna Display Selection Thymidylate Synthase Affinity Peptides
学科领域	Biochemistry & Molecular Biology ; Biophysics
收录类别	SCI
语种	英语
WOS记录号	WOS:000233534300013
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3148
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Yale Univ, Sch Med, Dept Med & Pharmacol, New Haven, CT 06520 USA
推荐引用方式 GB/T 7714	Yan, S,Niu, RL,Zhang, PJ,et al. Identification of peptides that bind with thymidylate synthase RNA using mRNA display technique[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2005,32(11):1081-1087.
APA	Yan, S,Niu, RL,Zhang, PJ,&Lin, XK.(2005).Identification of peptides that bind with thymidylate synthase RNA using mRNA display technique.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,32(11),1081-1087.
MLA	Yan, S,et al."Identification of peptides that bind with thymidylate synthase RNA using mRNA display technique".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 32.11(2005):1081-1087.

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