One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata

doi:10.1016/j.jbiotec.2004.09.017

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	One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata
	Liu, LN ; Chen, XL ; Zhang, XY; Zhang, YZ ; Zhou, BC
	2005-03-02
发表期刊	JOURNAL OF BIOTECHNOLOGY
ISSN	0168-1656
卷号	116 期号:1 页码:91-100
文章类型	Article
摘要	Phycoerythrins have been widely used in food, cosmetics., immunodiagnostics and analytical reagents. An efficient one-step chromatography method for purification of R-phycoerythrins from Polysiphonia urceolata was described in this paper. Pure R-phycoerythrin was obtained with an absorbance ratio A(565)/A(280) of 5.6 and a high recovery yield of 67-33%, using a DEAE-Sepharose Fast Flow chromatography with a gradient elution of pH, alternative to common gradient elution of ionic strength. The absorption spectrum of R-phycoerythrin was characterized with three absorbance maxima at 565, 539 and 498 mum, respectively and the fluorescence emission spectrum at room temperature was measured to be 580nm. The results of native-PAGE. and SDS-PAGE showed no contamination by other proteins in the phycoerythrin solution. which suggests an efficient method for the separation and purification of R-phycoerythrins from Polysiphonia urceolata. (C) 2004 Elsevier B.V. All rights reserved.; Phycoerythrins have been widely used in food, cosmetics., immunodiagnostics and analytical reagents. An efficient one-step chromatography method for purification of R-phycoerythrins from Polysiphonia urceolata was described in this paper. Pure R-phycoerythrin was obtained with an absorbance ratio A(565)/A(280) of 5.6 and a high recovery yield of 67-33%, using a DEAE-Sepharose Fast Flow chromatography with a gradient elution of pH, alternative to common gradient elution of ionic strength. The absorption spectrum of R-phycoerythrin was characterized with three absorbance maxima at 565, 539 and 498 mum, respectively and the fluorescence emission spectrum at room temperature was measured to be 580nm. The results of native-PAGE. and SDS-PAGE showed no contamination by other proteins in the phycoerythrin solution. which suggests an efficient method for the separation and purification of R-phycoerythrins from Polysiphonia urceolata. (C) 2004 Elsevier B.V. All rights reserved.
关键词	Polysiphonia Urceolata R-phycoerythrin Separation Purification Ion-exchange Chromatography
学科领域	Biotechnology & Applied Microbiology
DOI	10.1016/j.jbiotec.2004.09.017
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000226711200008
引用统计	被引频次：111[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3110
专题	实验海洋生物学重点实验室
作者单位	1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu, LN,Chen, XL,Zhang, XY,et al. One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata[J]. JOURNAL OF BIOTECHNOLOGY,2005,116(1):91-100.
APA	Liu, LN,Chen, XL,Zhang, XY,Zhang, YZ,&Zhou, BC.(2005).One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata.JOURNAL OF BIOTECHNOLOGY,116(1),91-100.
MLA	Liu, LN,et al."One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata".JOURNAL OF BIOTECHNOLOGY 116.1(2005):91-100.

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