Four viral proteins of white spot syndrome virus (WSSV) that attach to shrimp cell membranes

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	Four viral proteins of white spot syndrome virus (WSSV) that attach to shrimp cell membranes
	Liang, Y ; Huang, J ; Song, XL ; Zhang, PJ ; Xu, HS
	2005-08-09
发表期刊	DISEASES OF AQUATIC ORGANISMS
ISSN	0177-5103
卷号	66 期号:1 页码:81-85
文章类型	Article
摘要	White spot syndrome virus (WSSV) is a major shrimp pathogen that has a widespread negative affect on shrimp production in Asia and the Americas. It is known that WSSV infects shrimp cells through viral attachment proteins (VAP) that bind with shrimp cell receptors. However, the identity of both WSSV VAP and shrimp cell receptors remains unclear. We used digoxigenin (DIG)labeled shrimp hemocyte and gill cell membranes to bind to WSSV proteins immobilized on nitrocellulose membranes, and 4 putative WSSV VAP (37 kDa, 39 kDa and 2 above 97 kDa) were identified. Mass spectrometric analysis identified the 37 kDa putative VAP as the product of WSSV gene VP281.; White spot syndrome virus (WSSV) is a major shrimp pathogen that has a widespread negative affect on shrimp production in Asia and the Americas. It is known that WSSV infects shrimp cells through viral attachment proteins (VAP) that bind with shrimp cell receptors. However, the identity of both WSSV VAP and shrimp cell receptors remains unclear. We used digoxigenin (DIG)labeled shrimp hemocyte and gill cell membranes to bind to WSSV proteins immobilized on nitrocellulose membranes, and 4 putative WSSV VAP (37 kDa, 39 kDa and 2 above 97 kDa) were identified. Mass spectrometric analysis identified the 37 kDa putative VAP as the product of WSSV gene VP281.
关键词	Viral Attachment Protein White Spot Syndrome Virus Wssv Shrimp Vp28
学科领域	Fisheries ; Veterinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000232577300010
引用统计	被引频次：36[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3100
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Ocean Univ China, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Liang, Y,Huang, J,Song, XL,et al. Four viral proteins of white spot syndrome virus (WSSV) that attach to shrimp cell membranes[J]. DISEASES OF AQUATIC ORGANISMS,2005,66(1):81-85.
APA	Liang, Y,Huang, J,Song, XL,Zhang, PJ,&Xu, HS.(2005).Four viral proteins of white spot syndrome virus (WSSV) that attach to shrimp cell membranes.DISEASES OF AQUATIC ORGANISMS,66(1),81-85.
MLA	Liang, Y,et al."Four viral proteins of white spot syndrome virus (WSSV) that attach to shrimp cell membranes".DISEASES OF AQUATIC ORGANISMS 66.1(2005):81-85.

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