The genomic structure, alternative splicing and immune response of Chlamys farreri thioester-containing protein

doi:10.1016/j.dci.2009.05.007

IOCAS-IR > 实验海洋生物学重点实验室

	The genomic structure, alternative splicing and immune response of Chlamys farreri thioester-containing protein
	Zhang, Huan 1,2; Wang, Lingling 1; Song, Linsheng 1; Zhao, Jianmin 1; Qiu, Limei 1; Gao, Yang1,2 ; Song, Xiaoyan 1; Li, Ling 1,2; Zhang, Ying 1,2; Zhang, Lei 1
	2009-10-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	33 期号:10 页码:1070-1076
文章类型	Article
摘要	MEP is a member of thioester-containing protein (TEP) family found in Zhikong scallop Chlamys farreri and is involved in innate immunity against invading microbes. In the present study, the genomic DNA of CfTEP was cloned and characterized. The genomic DNA sequence of CfTEP consisted of 40 exons and 39 introns spanning 35 kb with all exon-intron junction sequences agreeing with the GT/AG consensus. The genomic organization of CfTEP was similar to human and mouse 0 rather than ciona C3-1 and Drosophila dTEP2. By RT-PCR technique, seven different cDNA variants of CfTEP (designated as CfTEP-A-CfTEP-G) were cloned from scallop gonad. CfTEP-A-CfTEP-F were produced by alternative splicing of six mutually exclusive exons (exons 19-24), respectively, which encoded the highly variable central region. While in CfTEP-G, the deletion of all the six exons introduced a new translation stop site and might trigger nonsense mediated decay (NMD). The mRNA expression and the proportion of the seven CfTEP variant transcripts were examined in the gonad of scallops after bacterial challenge. The fragments containing the highly variable central region of UTEP were amplified by RT-PCR and a 100 positive clones were sequenced randomly. The expression profiles of the seven MEP variants were different and displayed the sex and bacteria dependent manner. In the blank, sea water and Listonella anguillarum challenged subgroups of male scallops, all the transcripts detected were CfTEP-G isoform. In the Micrococcus luteus challenged subgroup, the isoforms expressed and their proportions were CfTEP-F (54%), CfTEP-B (23%), CfTEP-A (10%), CfTEP-C (7%) and CfTEP-E (6%). However, in the gonad of female scallops, only CfTEP-A were found in blank and sea water challenged subgroups. After L anguillarum or M. luteus challenge, four and five isoforms were detected, respectively, with CfTEP-F isoform being the most one in the both subgroups. These results suggested that the evolution of TEP genes was very complex, and that the diverse CfTEP transcripts generated by alternative splicing played an important role as pattern recognition receptors in the innate immune defense of scallops. (C) 2009 Elsevier Ltd. All rights reserved.; MEP is a member of thioester-containing protein (TEP) family found in Zhikong scallop Chlamys farreri and is involved in innate immunity against invading microbes. In the present study, the genomic DNA of CfTEP was cloned and characterized. The genomic DNA sequence of CfTEP consisted of 40 exons and 39 introns spanning 35 kb with all exon-intron junction sequences agreeing with the GT/AG consensus. The genomic organization of CfTEP was similar to human and mouse 0 rather than ciona C3-1 and Drosophila dTEP2. By RT-PCR technique, seven different cDNA variants of CfTEP (designated as CfTEP-A-CfTEP-G) were cloned from scallop gonad. CfTEP-A-CfTEP-F were produced by alternative splicing of six mutually exclusive exons (exons 19-24), respectively, which encoded the highly variable central region. While in CfTEP-G, the deletion of all the six exons introduced a new translation stop site and might trigger nonsense mediated decay (NMD). The mRNA expression and the proportion of the seven CfTEP variant transcripts were examined in the gonad of scallops after bacterial challenge. The fragments containing the highly variable central region of UTEP were amplified by RT-PCR and a 100 positive clones were sequenced randomly. The expression profiles of the seven MEP variants were different and displayed the sex and bacteria dependent manner. In the blank, sea water and Listonella anguillarum challenged subgroups of male scallops, all the transcripts detected were CfTEP-G isoform. In the Micrococcus luteus challenged subgroup, the isoforms expressed and their proportions were CfTEP-F (54%), CfTEP-B (23%), CfTEP-A (10%), CfTEP-C (7%) and CfTEP-E (6%). However, in the gonad of female scallops, only CfTEP-A were found in blank and sea water challenged subgroups. After L anguillarum or M. luteus challenge, four and five isoforms were detected, respectively, with CfTEP-F isoform being the most one in the both subgroups. These results suggested that the evolution of TEP genes was very complex, and that the diverse CfTEP transcripts generated by alternative splicing played an important role as pattern recognition receptors in the innate immune defense of scallops. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Chlamis Farreri Thioester-containing Protein (Tep) Gene Structure Alternative Splicing Nonsense Mediated Decay (Nmd) Immune Response
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2009.05.007
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000268952800004
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3080
专题	实验海洋生物学重点实验室海洋环境腐蚀与生物污损重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Huan,Wang, Lingling,Song, Linsheng,et al. The genomic structure, alternative splicing and immune response of Chlamys farreri thioester-containing protein[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2009,33(10):1070-1076.
APA	Zhang, Huan.,Wang, Lingling.,Song, Linsheng.,Zhao, Jianmin.,Qiu, Limei.,...&Zhang, Lei.(2009).The genomic structure, alternative splicing and immune response of Chlamys farreri thioester-containing protein.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,33(10),1070-1076.
MLA	Zhang, Huan,et al."The genomic structure, alternative splicing and immune response of Chlamys farreri thioester-containing protein".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 33.10(2009):1070-1076.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
zhang-The genomic st（326KB）			限制开放	--	浏览