Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli

doi:10.1042/BA20070187

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	Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli
	Zhang, Weijie 1,2; Guan, Xiangyu 3; Yang, Yu 1,2; Ge, Baosheng 4; Chen, Huaxin 1; Li, Fuchao 1; Qin, Song 1
	2009-02-01
发表期刊	BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
ISSN	0885-4513
卷号	52 页码:135-140
文章类型	Article
摘要	APC (allophycocyanin) is widely used for fluorescence tagging and may be a promising antioxidant agent for use within the food and pharmaceutical industries. Chromophore attachment to apo-ApcA (apo-APC alpha-subunit without chromophore) can be auto-catalysed both in vitro and in vivo. In the present study, a plasmid containing genes of apo-ApcA and chromophore synthetases (HOI (ferredoxin-dependent haem oxygenase) and PcyA (phycocyanobilin:ferredoxin oxidoreductase)] was constructed and expressed in Escherichia coli. The results show that holo-ApcA (APC alpha-subunit with chromophore) can be synthesized by autocatalysis in E. coli. Recombinant holo-ApcA showed the same spectral and fluorescent properties as PC (phycocyanin) and could serve as a good substitute for native PC for fluorescent tagging. Moreover, recombinant ApcA can inhibit hydroxyl and peroxyl radicals more strongly than holo-ApcA and native APC. The EC50 values were 296.4 +/- 22.4 mu g/ml against hydroxyl radicals and 38.5 +/- 2.6 mu g/ml against peroxyl radicals.; APC (allophycocyanin) is widely used for fluorescence tagging and may be a promising antioxidant agent for use within the food and pharmaceutical industries. Chromophore attachment to apo-ApcA (apo-APC alpha-subunit without chromophore) can be auto-catalysed both in vitro and in vivo. In the present study, a plasmid containing genes of apo-ApcA and chromophore synthetases (HOI (ferredoxin-dependent haem oxygenase) and PcyA (phycocyanobilin:ferredoxin oxidoreductase)] was constructed and expressed in Escherichia coli. The results show that holo-ApcA (APC alpha-subunit with chromophore) can be synthesized by autocatalysis in E. coli. Recombinant holo-ApcA showed the same spectral and fluorescent properties as PC (phycocyanin) and could serve as a good substitute for native PC for fluorescent tagging. Moreover, recombinant ApcA can inhibit hydroxyl and peroxyl radicals more strongly than holo-ApcA and native APC. The EC50 values were 296.4 +/- 22.4 mu g/ml against hydroxyl radicals and 38.5 +/- 2.6 mu g/ml against peroxyl radicals.
关键词	Autocatalysis Escherichia Coli Fluorescence Recombinant Apo-allophycocyanin Alpha-subunit Recombinant Holo-allophycocyanin Alpha-subunit
学科领域	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
DOI	10.1042/BA20070187
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000263202500005
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3070
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marin Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Ocean Univ China, Fac Life Sci & Technol, Coll Marine Life Sci, Qingdao 266003, Peoples R China 4.China Univ Petr, Ctr Biotechnol & Bioengn, Qingdao 266555, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Weijie,Guan, Xiangyu,Yang, Yu,et al. Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2009,52:135-140.
APA	Zhang, Weijie.,Guan, Xiangyu.,Yang, Yu.,Ge, Baosheng.,Chen, Huaxin.,...&Qin, Song.(2009).Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,52,135-140.
MLA	Zhang, Weijie,et al."Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 52(2009):135-140.

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