Expressed sequence tags from the zhikong scallop (Chlamys farreri): Discovery and annotation of host-defense genes

doi:10.1016/j.fsi.2009.03.002

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	Expressed sequence tags from the zhikong scallop (Chlamys farreri): Discovery and annotation of host-defense genes
	Wang, Lingling 1; Song, Linsheng 1; Zhao, Jianmin 1; Qiu, Limei 1; Zhang, Huan 1; Xu, Wei 1; Li, Honglei1 ; Li, Chenhua 1; Wu, Longtao 1; Guo, Ximing 2
	2009-05-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	26 期号:5 页码:744-750
文章类型	Article
摘要	A high-quality cDNA library was constructed from whole body tissues of the zhikong scallop, Chlamys farreri, challenged by Listonella anguillarum. A total of 5720 clones were sequenced, yielding 5123 expressed sequence tags (ESTs). Among the 3326 unique genes identified, 2289 (69%) genes had no significant (E-value < 1e-5) matches to known sequences in public databases and 194 (6%) matched proteins of unknown functions. The remaining 843 (25%) genes that exhibited homology with genes of known functions, showed broad involvement in metabolic processes (31%), cell structure and motility (20%), gene and protein expression (12%), cell signaling and cell communication (8%), cell division (4%), and notably, 25% of those genes were related to immune function. They included stress response genes, complement-like genes, proteinase and proteinase inhibitors, immune recognition receptors and immune effectors. The EST collection obtained in this study provides a useful resource for gene discovery and especially for the identification of host-defense genes and systems in scallops and other molluscs. (C) 2009 Elsevier Ltd. All rights reserved.; A high-quality cDNA library was constructed from whole body tissues of the zhikong scallop, Chlamys farreri, challenged by Listonella anguillarum. A total of 5720 clones were sequenced, yielding 5123 expressed sequence tags (ESTs). Among the 3326 unique genes identified, 2289 (69%) genes had no significant (E-value < 1e-5) matches to known sequences in public databases and 194 (6%) matched proteins of unknown functions. The remaining 843 (25%) genes that exhibited homology with genes of known functions, showed broad involvement in metabolic processes (31%), cell structure and motility (20%), gene and protein expression (12%), cell signaling and cell communication (8%), cell division (4%), and notably, 25% of those genes were related to immune function. They included stress response genes, complement-like genes, proteinase and proteinase inhibitors, immune recognition receptors and immune effectors. The EST collection obtained in this study provides a useful resource for gene discovery and especially for the identification of host-defense genes and systems in scallops and other molluscs. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Chlamys Farreri Cdna Library Expressed Sequence Tags Bacteria Challenge Functional Annotation Host-defense Genes
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2009.03.002
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000266659400010
引用统计	被引频次：61[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3032
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Lingling,Song, Linsheng,Zhao, Jianmin,et al. Expressed sequence tags from the zhikong scallop (Chlamys farreri): Discovery and annotation of host-defense genes[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(5):744-750.
APA	Wang, Lingling.,Song, Linsheng.,Zhao, Jianmin.,Qiu, Limei.,Zhang, Huan.,...&Guo, Ximing.(2009).Expressed sequence tags from the zhikong scallop (Chlamys farreri): Discovery and annotation of host-defense genes.FISH & SHELLFISH IMMUNOLOGY,26(5),744-750.
MLA	Wang, Lingling,et al."Expressed sequence tags from the zhikong scallop (Chlamys farreri): Discovery and annotation of host-defense genes".FISH & SHELLFISH IMMUNOLOGY 26.5(2009):744-750.

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