Immunoprotective analysis of VhhP2, a Vibrio harveyi vaccine candidate

doi:10.1016/j.vaccine.2009.03.012

IOCAS-IR > 实验海洋生物学重点实验室

	Immunoprotective analysis of VhhP2, a Vibrio harveyi vaccine candidate
	Sun, Kun 1,2; Zhang, Wei-wei 1,2; Hou, Jin-hui 3; Sun, Li 1
	2009-05-11
发表期刊	VACCINE
ISSN	0264-410X
卷号	27 期号:21 页码:2733-2740
文章类型	Article
摘要	VhhP2 is an Outer membrane protein identified in a pathogenic Vibrio harveyi strain, T4, isolated from diseased fish. When used as a Subunit Vaccine, purified recombinant VhhP2 affords high level of protection upon Japanese flounder against V harveyi challenge. Vaccination with VhhP2 induced the expression of a number of immune-related genes, especially those encoding immunoglobulin M (IgM) and major histocompatibility complex (MHC) II alpha. A VhhP2 surface display system, in the form of the fish commensal strain FIR harboring the vhhP2-expressing plasmid pJVP, was constructed. PF3/pJVP is able to produce and present recombinant VhhP2 on cell surface. Vaccination of fish with live PF3/pJVP via intraperitoneal injection elicited Strong immunoprotection. Vaccination of fish orally with live PF3/pJVP embedded in alginate microspheres also induced effective immunoprotection. In addition, a VhhP2-based surface display system was created, in which VhhP2 serves as a carrier for the Surface delivery of a heterologous Edwardsiella tarda immunogen, Et18, that is fused in-frame to VhhP2. DH5 alpha/pJVP18, which expresses and surface-displays the VhhP2-Et18 chimera, proved to be an effective vaccine that call protect fish against infections by V. harveyi and E. tarda to the extents comparable to those produced by vaccination with purified recombinant VhhP2 and Et18, respectively. These data suggest that VhhP2 may be applied as a vaccine and a vaccine carrier against infections by V. harveyi and other pathogens such as F. tarda. (C) 2009 Elsevier Ltd. All rights reserved.; VhhP2 is an Outer membrane protein identified in a pathogenic Vibrio harveyi strain, T4, isolated from diseased fish. When used as a Subunit Vaccine, purified recombinant VhhP2 affords high level of protection upon Japanese flounder against V harveyi challenge. Vaccination with VhhP2 induced the expression of a number of immune-related genes, especially those encoding immunoglobulin M (IgM) and major histocompatibility complex (MHC) II alpha. A VhhP2 surface display system, in the form of the fish commensal strain FIR harboring the vhhP2-expressing plasmid pJVP, was constructed. PF3/pJVP is able to produce and present recombinant VhhP2 on cell surface. Vaccination of fish with live PF3/pJVP via intraperitoneal injection elicited Strong immunoprotection. Vaccination of fish orally with live PF3/pJVP embedded in alginate microspheres also induced effective immunoprotection. In addition, a VhhP2-based surface display system was created, in which VhhP2 serves as a carrier for the Surface delivery of a heterologous Edwardsiella tarda immunogen, Et18, that is fused in-frame to VhhP2. DH5 alpha/pJVP18, which expresses and surface-displays the VhhP2-Et18 chimera, proved to be an effective vaccine that call protect fish against infections by V. harveyi and E. tarda to the extents comparable to those produced by vaccination with purified recombinant VhhP2 and Et18, respectively. These data suggest that VhhP2 may be applied as a vaccine and a vaccine carrier against infections by V. harveyi and other pathogens such as F. tarda. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Cross-protection Immune Response Surface Display Vaccine Vibrio Harveyi
学科领域	Immunology ; Medicine, Research & Experimental
DOI	10.1016/j.vaccine.2009.03.012
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000266023100002
引用统计	被引频次：97[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3018
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Xuzhou Inst Technol, Xuzhou, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Kun,Zhang, Wei-wei,Hou, Jin-hui,et al. Immunoprotective analysis of VhhP2, a Vibrio harveyi vaccine candidate[J]. VACCINE,2009,27(21):2733-2740.
APA	Sun, Kun,Zhang, Wei-wei,Hou, Jin-hui,&Sun, Li.(2009).Immunoprotective analysis of VhhP2, a Vibrio harveyi vaccine candidate.VACCINE,27(21),2733-2740.
MLA	Sun, Kun,et al."Immunoprotective analysis of VhhP2, a Vibrio harveyi vaccine candidate".VACCINE 27.21(2009):2733-2740.

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