Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01

doi:10.1007/s00343-009-9216-x

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	Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01
	Qiao Hongjin 1,2; Wang Guangce 1,3
	2009-11-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	27 期号:4 页码:762-768
文章类型	Article
摘要	Heterotrophic culture of microalgae to develop methods of increasing biomass productivity and storage lipids has brought new insight to commercial biodiesel production. To understand the relationship between heterotrophy and lipid production, the effects of carbon sources on the growth and lipid accumulation of Chlorella sorokiniana GXNN01 was studied. The alga exhibited an increased growth rate in response to the addition of carbon sources, which reached the stationary phase after 48 h at 30A degrees C. In addition, glucose and NaAc had a significant effect on the lipid accumulation during the early-stationary phase. Specifically, the lipid content was 0.237 +/- 0.026 g g(-1) cell dry weight and 0.272 +/- 0.041 g L(-1) when glucose was used as the carbon source, whereas the lipid content reached 0.287 +/- 0.018 g g(-1) cell dry weight and 0.288 +/- 0.008 g L(-1) when NaAc was used as the carbon source. The neutral lipid content was found to first decrease and then increase over time during the growth phase. A glucose concentration of 20 mmol L(-1) gave the maximal lipid yield and the optimum harvest time was the early-stationary phase.; Heterotrophic culture of microalgae to develop methods of increasing biomass productivity and storage lipids has brought new insight to commercial biodiesel production. To understand the relationship between heterotrophy and lipid production, the effects of carbon sources on the growth and lipid accumulation of Chlorella sorokiniana GXNN01 was studied. The alga exhibited an increased growth rate in response to the addition of carbon sources, which reached the stationary phase after 48 h at 30A degrees C. In addition, glucose and NaAc had a significant effect on the lipid accumulation during the early-stationary phase. Specifically, the lipid content was 0.237 +/- 0.026 g g(-1) cell dry weight and 0.272 +/- 0.041 g L-1 when glucose was used as the carbon source, whereas the lipid content reached 0.287 +/- 0.018 g g(-1) cell dry weight and 0.288 +/- 0.008 g L-1 when NaAc was used as the carbon source. The neutral lipid content was found to first decrease and then increase over time during the growth phase. A glucose concentration of 20 mmol L-1 gave the maximal lipid yield and the optimum harvest time was the early-stationary phase.
关键词	Heterotrophic Microalgae Biodiesel Carbon Source Lipid
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-009-9216-x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000272422300010
引用统计	被引频次：55[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3002
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Tianjin Univ Sci & Technol, Tianjin 300222, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Qiao Hongjin,Wang Guangce. Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(4):762-768.
APA	Qiao Hongjin,&Wang Guangce.(2009).Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(4),762-768.
MLA	Qiao Hongjin,et al."Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.4(2009):762-768.

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