A novel anti-tumor protein extracted from Meretrix meretrix Linnaeus induces cell death by increasing cell permeability and inhibiting tubulin polymerization

doi:10.3892/ijo_00000393

IOCAS-IR > 实验海洋生物学重点实验室

	A novel anti-tumor protein extracted from Meretrix meretrix Linnaeus induces cell death by increasing cell permeability and inhibiting tubulin polymerization
	Ning, Xuanxuan 1,2; Zhao, Jianmin 1; Zhang, Yuyan 1; Cao, Subing 1; Liu, Ming 1; Ling, Peixue 3; Lin, Xiukun 1
	2009-10-01
发表期刊	INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN	1019-6439
卷号	35 期号:4 页码:805-812
文章类型	Article
摘要	Discovery and development of new pharmaceuticals from marine organisms are attracting increasing interest. Several agents derived from marine organisms are under preclinical and clinical evaluation as potential anticancer drugs. We extracted and purified a novel anti-tumor protein from the coelomic fluid of Meretrix meretrix Linnaeus by ammonium sulphate fractionation, ion exchange and hydrophobic interaction chromatography. The molecular weight of the highly purified protein, designated MML, was 40 kDa as determined by SDS-PAGE analysis. MML exhibited significant cytotoxicity to several cancer cell types, including human hepatoma BEL-7402, human breast cancer MCF-7 and human colon cancer HCT116 cells. However, no inhibitory effect was found when treating murine normal fibroblasts NIH3T3 and benign human breast MCF-10A cells with MML. The cell death induced by MML was characterized by cell morphological changes. The induction of apoptosis of BEL-7402 cells by MML was weak by DNA ladder assay. The possible mechanisms of its anti-tumor effect might be the changes in cell membrane permeability and inhibition of tubulin polymerization. MML may be developed as a novel, highly selective and effective anti-cancer drug.; Discovery and development of new pharmaceuticals from marine organisms are attracting increasing interest. Several agents derived from marine organisms are under preclinical and clinical evaluation as potential anticancer drugs. We extracted and purified a novel anti-tumor protein from the coelomic fluid of Meretrix meretrix Linnaeus by ammonium sulphate fractionation, ion exchange and hydrophobic interaction chromatography. The molecular weight of the highly purified protein, designated MML, was 40 kDa as determined by SDS-PAGE analysis. MML exhibited significant cytotoxicity to several cancer cell types, including human hepatoma BEL-7402, human breast cancer MCF-7 and human colon cancer HCT116 cells. However, no inhibitory effect was found when treating murine normal fibroblasts NIH3T3 and benign human breast MCF-10A cells with MML. The cell death induced by MML was characterized by cell morphological changes. The induction of apoptosis of BEL-7402 cells by MML was weak by DNA ladder assay. The possible mechanisms of its anti-tumor effect might be the changes in cell membrane permeability and inhibition of tubulin polymerization. MML may be developed as a novel, highly selective and effective anti-cancer drug.
关键词	Meretrix Meretrix Linnaeus Anti-tumor Protein Cytotoxicity Cell Membrane Permeability Cell Cycle Tubulin Polymerization Apoptosis
学科领域	Oncology
DOI	10.3892/ijo_00000393
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000269780600016
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2994
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Yantai Ocean Environm Monitoring Cent Stn SOA, Yantai 264006, Peoples R China 3.Inst Biopharmaceut Shandong Prov, Jinan 250012, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ning, Xuanxuan,Zhao, Jianmin,Zhang, Yuyan,et al. A novel anti-tumor protein extracted from Meretrix meretrix Linnaeus induces cell death by increasing cell permeability and inhibiting tubulin polymerization[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2009,35(4):805-812.
APA	Ning, Xuanxuan.,Zhao, Jianmin.,Zhang, Yuyan.,Cao, Subing.,Liu, Ming.,...&Lin, Xiukun.(2009).A novel anti-tumor protein extracted from Meretrix meretrix Linnaeus induces cell death by increasing cell permeability and inhibiting tubulin polymerization.INTERNATIONAL JOURNAL OF ONCOLOGY,35(4),805-812.
MLA	Ning, Xuanxuan,et al."A novel anti-tumor protein extracted from Meretrix meretrix Linnaeus induces cell death by increasing cell permeability and inhibiting tubulin polymerization".INTERNATIONAL JOURNAL OF ONCOLOGY 35.4(2009):805-812.

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