Characterization of an ETS transcription factor in the sea scallop Chlamys farreri

doi:10.1016/j.dci.2009.05.003

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	Characterization of an ETS transcription factor in the sea scallop Chlamys farreri
	Ma, Hongming 1,2; Wang, Jia; Wang, Bing 2; Zhao, Yancui ; Yang, Changjian 2
	2009-09-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	33 期号:9 页码:953-958
文章类型	Article
摘要	We have cloned and characterized a cDNA encoding a putative ETS transcription factor, designated Cf-ets. The Cf-ets encodes a 406 amino acid protein containing a conserved ETS domain and a Pointed domain. Phylogenetic analysis revealed that Cf-ets belongs to the ESE group of ETS transcription factor family. Real-time PCR analysis of Cf-ets expression in adult sea scallop tissues revealed that Cf-ets was expressed mainly in gill and hemocytes, in a constitutive manner. Cf-ets mRNA level in hemocytes increased drastically after microbial challenge indicated its indispensable role in the anti-infection process. Simultaneously, the circulating hemocyte number decreased. In mammals, most ETS transcription factors play indispensable roles in blood cell differentiation and linage commitment during hematopoisis. Cf-ets is therefore likely to be a potential biomarker for hematopoiesis studies in scallops. (C) 2009 Elsevier Ltd. All rights reserved.; We have cloned and characterized a cDNA encoding a putative ETS transcription factor, designated Cf-ets. The Cf-ets encodes a 406 amino acid protein containing a conserved ETS domain and a Pointed domain. Phylogenetic analysis revealed that Cf-ets belongs to the ESE group of ETS transcription factor family. Real-time PCR analysis of Cf-ets expression in adult sea scallop tissues revealed that Cf-ets was expressed mainly in gill and hemocytes, in a constitutive manner. Cf-ets mRNA level in hemocytes increased drastically after microbial challenge indicated its indispensable role in the anti-infection process. Simultaneously, the circulating hemocyte number decreased. In mammals, most ETS transcription factors play indispensable roles in blood cell differentiation and linage commitment during hematopoisis. Cf-ets is therefore likely to be a potential biomarker for hematopoiesis studies in scallops. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Cloning Immune Challenge Hematopoiesis Mollusk Suppressive Subtractive Hybridization Hemocyte Count Phylogeny Real-time Pcr
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2009.05.003
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000268088800001
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2988
专题	实验海洋生物学重点实验室
作者单位	1.Ocean Univ China, Food Sci & Technol Coll, Minist Educ China, Key Lab Mariculture, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Ma, Hongming,Wang, Jia,Wang, Bing,et al. Characterization of an ETS transcription factor in the sea scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2009,33(9):953-958.
APA	Ma, Hongming,Wang, Jia,Wang, Bing,Zhao, Yancui,&Yang, Changjian.(2009).Characterization of an ETS transcription factor in the sea scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,33(9),953-958.
MLA	Ma, Hongming,et al."Characterization of an ETS transcription factor in the sea scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 33.9(2009):953-958.

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