Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates

doi:10.1016/j.cryobiol.2009.04.004

IOCAS-IR > 实验海洋生物学重点实验室

	Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates
	Li, J.1 ; Zhang, L. L.1,2; Liu, Q. H.1,2; Xu, X. Z.1,2; Xiao, Z. Z.1; Ma, D. Y.1; Xu, S. H.1; Xue, Q. Z.3
	2009-08-01
发表期刊	CRYOBIOLOGY
ISSN	0011-2240
卷号	59 期号:1 页码:48-53
文章类型	Article
摘要	The ice crystal formation is assumed as the most lethal factor for the failure of fish embryo cryopreservation and intracellular ice formation (IIF) plays a central role in cell injury during cooling. The objectives were to observe the morphological changes of red seabream (Pagrus major) embryo during the cooling-thawing process, and to examine the effect of cryoprotectant and cooling rate on the temperatures of oil globule ice formation (T-OIF), extra-cellular ice formation (T-EIF) and intracellular ice formation (T-IIF) using cryomicroscope. After thawing, the morphological changes of embryos were observed and recorded by the video attachment and monitor under the microscope. During the cooling process, three representative phenomena were observed: oil globule gradually turned bright firstly, then the whole field of view flashed and the embryo blackened. Cooling rate affect the temperature of both extra- and intra-cellular ice formations. T-EIF and T-IIF at high cooling rate were much lower than that at low cooling rate. And the value of T-EIF - T-IIF increased from 0.45 to 11.11 degrees C with the increase of cooling rate from 3 to 130 degrees C/min. Taken together, our results suggested that high cooling rate with proper cryoprotectant would be a good option for red seabream embryo cryopreservation. (C) 2009 Elsevier Inc. All rights reserved.; The ice crystal formation is assumed as the most lethal factor for the failure of fish embryo cryopreservation and intracellular ice formation (IIF) plays a central role in cell injury during cooling. The objectives were to observe the morphological changes of red seabream (Pagrus major) embryo during the cooling-thawing process, and to examine the effect of cryoprotectant and cooling rate on the temperatures of oil globule ice formation (T-OIF), extra-cellular ice formation (T-EIF) and intracellular ice formation (T-IIF) using cryomicroscope. After thawing, the morphological changes of embryos were observed and recorded by the video attachment and monitor under the microscope. During the cooling process, three representative phenomena were observed: oil globule gradually turned bright firstly, then the whole field of view flashed and the embryo blackened. Cooling rate affect the temperature of both extra- and intra-cellular ice formations. T-EIF and T-IIF at high cooling rate were much lower than that at low cooling rate. And the value of T-EIF - T-IIF increased from 0.45 to 11.11 degrees C with the increase of cooling rate from 3 to 130 degrees C/min. Taken together, our results suggested that high cooling rate with proper cryoprotectant would be a good option for red seabream embryo cryopreservation. (C) 2009 Elsevier Inc. All rights reserved.
关键词	Red Seabream Embryo Extra- And Intra-cellular Ice Formation Cooling Rate
学科领域	Biology ; Physiology
DOI	10.1016/j.cryobiol.2009.04.004
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000268059000008
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2950
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, J.,Zhang, L. L.,Liu, Q. H.,et al. Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates[J]. CRYOBIOLOGY,2009,59(1):48-53.
APA	Li, J..,Zhang, L. L..,Liu, Q. H..,Xu, X. Z..,Xiao, Z. Z..,...&Xue, Q. Z..(2009).Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates.CRYOBIOLOGY,59(1),48-53.
MLA	Li, J.,et al."Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates".CRYOBIOLOGY 59.1(2009):48-53.

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