Assembly of the subcellular parts of Bryopsis hypnoides Lamouroux into new protoplasts

doi:10.1134/S1021443709010166

IOCAS-IR > 实验海洋生物学重点实验室

	Assembly of the subcellular parts of Bryopsis hypnoides Lamouroux into new protoplasts
	Li, D.1,3,4; Lue, F.1,4; Wang, G.1,2; Zhou, B.4
	2009
发表期刊	RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
ISSN	1021-4437
卷号	56 期号:1 页码:110-117
文章类型	Article
摘要	The chloroplasts, mitochondria, and protoplasm devoid of mature chloroplasts (PMC) of Bryopsis hypnoides Lamouroux were isolated by low-speed and sucrose density centrifugation. The PMC aggregated in artificial seawater, and then protoplasts without mature chloroplasts (PtMCs) were formed. Transmission electron microscopy and cytochemical studies indicated that there were mitochondria, nuclei, vesicles, and other small cell organelles in the PtMCs. Scanning electron microscopy showed that there were holes on the surface of 1-h PtMCs and then fewer holes on the surface of 24-h PtMCs, suggesting that a healing process occurred. The plasma membrane was formed over the surface of the PtMCs. However, the cell wall was not regenerated, and the newly formed PtMCs were ruptured and died in 3 days. Light intensity during alga maintenance before use influenced significantly (one-way ANOVA, P < 0.0001) on the number of PtMCs formed; the highest number of PtMCs was formed at 20A mu mol/(m(2) s). When isolated chloroplasts were transferred into seawater, there were only two or three chloroplasts aggregated together. However, isolated mitochondria and the mixed six layers of cell organelles (separated by sucrose density centrifugation) could not aggregate in the artificial seawater. This indicates that the conjunction of cell organelles is important for their aggregation.; The chloroplasts, mitochondria, and protoplasm devoid of mature chloroplasts (PMC) of Bryopsis hypnoides Lamouroux were isolated by low-speed and sucrose density centrifugation. The PMC aggregated in artificial seawater, and then protoplasts without mature chloroplasts (PtMCs) were formed. Transmission electron microscopy and cytochemical studies indicated that there were mitochondria, nuclei, vesicles, and other small cell organelles in the PtMCs. Scanning electron microscopy showed that there were holes on the surface of 1-h PtMCs and then fewer holes on the surface of 24-h PtMCs, suggesting that a healing process occurred. The plasma membrane was formed over the surface of the PtMCs. However, the cell wall was not regenerated, and the newly formed PtMCs were ruptured and died in 3 days. Light intensity during alga maintenance before use influenced significantly (one-way ANOVA, P < 0.0001) on the number of PtMCs formed; the highest number of PtMCs was formed at 20A mu mol/(m(2) s). When isolated chloroplasts were transferred into seawater, there were only two or three chloroplasts aggregated together. However, isolated mitochondria and the mixed six layers of cell organelles (separated by sucrose density centrifugation) could not aggregate in the artificial seawater. This indicates that the conjunction of cell organelles is important for their aggregation.
关键词	Bryopsis Hypnoides Assembly Cell Membrane Chloroplast Mitochondria Protoplast
学科领域	Plant Sciences
DOI	10.1134/S1021443709010166
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000262485000016
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2944
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 3.Liaocheng Univ, Inst Agr, Dept Food Sci, Liaocheng 252059, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, D.,Lue, F.,Wang, G.,et al. Assembly of the subcellular parts of Bryopsis hypnoides Lamouroux into new protoplasts[J]. RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,2009,56(1):110-117.
APA	Li, D.,Lue, F.,Wang, G.,&Zhou, B..(2009).Assembly of the subcellular parts of Bryopsis hypnoides Lamouroux into new protoplasts.RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,56(1),110-117.
MLA	Li, D.,et al."Assembly of the subcellular parts of Bryopsis hypnoides Lamouroux into new protoplasts".RUSSIAN JOURNAL OF PLANT PHYSIOLOGY 56.1(2009):110-117.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
li-Assembly of the s（435KB）			限制开放	--	浏览