Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain

doi:10.1007/s12275-008-0278-9

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	Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain
	Hu, Yong-hua 1,2; Wang, Hua-lei 3; Zhang, Min 1,2; Sun, Li 1
	2009-06-01
发表期刊	JOURNAL OF MICROBIOLOGY
ISSN	1225-8873
卷号	47 期号:3 页码:277-286
文章类型	Article
摘要	CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.; CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.
关键词	Copabcd Copper Resistance Copr Pseudomonas Fluorescens Virulence
学科领域	Microbiology
DOI	10.1007/s12275-008-0278-9
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000267396900008
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2920
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Hang Zhou EnzySource Biotechnol Co Ltd, Hangzhou 310030, Zhejiang, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hu, Yong-hua,Wang, Hua-lei,Zhang, Min,et al. Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain[J]. JOURNAL OF MICROBIOLOGY,2009,47(3):277-286.
APA	Hu, Yong-hua,Wang, Hua-lei,Zhang, Min,&Sun, Li.(2009).Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain.JOURNAL OF MICROBIOLOGY,47(3),277-286.
MLA	Hu, Yong-hua,et al."Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain".JOURNAL OF MICROBIOLOGY 47.3(2009):277-286.

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