The immune responses in Chinese mitten crab Eriocheir sinensis challenged with double-stranded RNA

doi:10.1016/j.fsi.2009.01.005

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	The immune responses in Chinese mitten crab Eriocheir sinensis challenged with double-stranded RNA
	Dong, Chaohua 1,2; Zhao, Jianmin 1; Song, Linsheng 1; Wang, Lingling 1; Qiu, Limei 1; Zheng, Peilin 1,3; Li, Ling 1,3; Gai, Yuncao 1,3; Yang, Guanpin 2
	2009-03-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	26 期号:3 页码:438-442
文章类型	Article
摘要	Double-stranded RNA (dsRNA) is a virus-associated molecular pattern which induces antiviral innate immune responses and RNA interference (RNAi) in mammals. In invertebrates, RNAi phenomenon has been widely studied, but dsRNA-induced innate immune response is seldom reported. In the present study, two different dsRNAs specific for green fluorescent protein (GFP) and the putative D1 protein of photosystem II (NoPSD) from Nannochloropsis oculata, were employed to challenge Chinese mitten crab Eriocheir sinensis. The temporal changes of phenoloxidase (PO), acid phosphatase (ACP), superoxide dismutase (SOD) and malondialdehyde (MDA) content, as well as the mRNA expression of some immune-related genes were examined in order to estimate the effect of dsRNAs on the innate immunity of E. sinensis. The activities of PO, ACP and SOD significantly increased after dsRNA treatment, whereas malondialdehyde (MDA) content did not change significantly. Among the examined genes, only the mRNA expression of EsALF, an antibacterial peptide in E. sinensis, was significantly up-regulated (about 5 fold, P < 0.05) at 12 h after dsRNA treatment, while no significant expression changes were observed among the other immune genes. The increase of PO, ACP and SOD activities, and mRNA expression level of EsALF after dsRNA stimulation indicate that phenoloxidase, hydrolytic enzyme, antioxidation and EsALF were involved in dsRNA-induced innate immunity, suggesting that broad-spectrum immune responses could be induced by dsRNA in E. sinensis. (C) 2009 Elsevier Ltd. All rights reserved.; Double-stranded RNA (dsRNA) is a virus-associated molecular pattern which induces antiviral innate immune responses and RNA interference (RNAi) in mammals. In invertebrates, RNAi phenomenon has been widely studied, but dsRNA-induced innate immune response is seldom reported. In the present study, two different dsRNAs specific for green fluorescent protein (GFP) and the putative D1 protein of photosystem II (NoPSD) from Nannochloropsis oculata, were employed to challenge Chinese mitten crab Eriocheir sinensis. The temporal changes of phenoloxidase (PO), acid phosphatase (ACP), superoxide dismutase (SOD) and malondialdehyde (MDA) content, as well as the mRNA expression of some immune-related genes were examined in order to estimate the effect of dsRNAs on the innate immunity of E. sinensis. The activities of PO, ACP and SOD significantly increased after dsRNA treatment, whereas malondialdehyde (MDA) content did not change significantly. Among the examined genes, only the mRNA expression of EsALF, an antibacterial peptide in E. sinensis, was significantly up-regulated (about 5 fold, P < 0.05) at 12 h after dsRNA treatment, while no significant expression changes were observed among the other immune genes. The increase of PO, ACP and SOD activities, and mRNA expression level of EsALF after dsRNA stimulation indicate that phenoloxidase, hydrolytic enzyme, antioxidation and EsALF were involved in dsRNA-induced innate immunity, suggesting that broad-spectrum immune responses could be induced by dsRNA in E. sinensis. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Eriocheir Sinensis Double-stranded Rna Immune Response Esalf Superoxide Dismutase Malondialdehyde Phenoloxidase Acid Phosphatase
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2009.01.005
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000265518300012
引用统计	被引频次：48[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2900
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Dong, Chaohua,Zhao, Jianmin,Song, Linsheng,et al. The immune responses in Chinese mitten crab Eriocheir sinensis challenged with double-stranded RNA[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(3):438-442.
APA	Dong, Chaohua.,Zhao, Jianmin.,Song, Linsheng.,Wang, Lingling.,Qiu, Limei.,...&Yang, Guanpin.(2009).The immune responses in Chinese mitten crab Eriocheir sinensis challenged with double-stranded RNA.FISH & SHELLFISH IMMUNOLOGY,26(3),438-442.
MLA	Dong, Chaohua,et al."The immune responses in Chinese mitten crab Eriocheir sinensis challenged with double-stranded RNA".FISH & SHELLFISH IMMUNOLOGY 26.3(2009):438-442.

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