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Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor | |
Deng, Xiangyuan1,2; Qin, Song1; Zhang, Qun1,2; Jiang, Peng1; Cui, Yulin1,2; Li, Xiaokun3 | |
2009-06-01 | |
发表期刊 | PLANT CELL TISSUE AND ORGAN CULTURE |
ISSN | 0167-6857 |
卷号 | 97期号:3页码:253-261 |
文章类型 | Article |
摘要 | A human acidic fibroblast growth factor gene, hafgf, was successfully transferred into Laminaria japonica (kelp) gametophytes via microprojectile bombardment using the biolistic PDS-1000/He gene gun. Following phosphinothricin screening, PCR detection and Southern blot analysis, transgenic L. japonica gametophytes were cultivated in an illuminated bubble-column bioreactor to optimize growth conditions. A maximal final dry cell density of 1,695 mg l(-1) was obtained in a batch culture having an initial dry cell density of 129.75 mg l(-1). This was achieved using an aeration rate of 1.08 l air min(-1) l(-1) culture in a medium containing 1.5 mM inorganic nitrate and 0.15 mM phosphate. In addition, the relationship between different nitrogen sources and growth of transgenic gametophytes indicated that both urea and sodium nitrate were effective nitrogen sources for cell growth, while ammonium ions inhibited growth of these gametophytes.; A human acidic fibroblast growth factor gene, hafgf, was successfully transferred into Laminaria japonica (kelp) gametophytes via microprojectile bombardment using the biolistic PDS-1000/He gene gun. Following phosphinothricin screening, PCR detection and Southern blot analysis, transgenic L. japonica gametophytes were cultivated in an illuminated bubble-column bioreactor to optimize growth conditions. A maximal final dry cell density of 1,695 mg l(-1) was obtained in a batch culture having an initial dry cell density of 129.75 mg l(-1). This was achieved using an aeration rate of 1.08 l air min(-1) l(-1) culture in a medium containing 1.5 mM inorganic nitrate and 0.15 mM phosphate. In addition, the relationship between different nitrogen sources and growth of transgenic gametophytes indicated that both urea and sodium nitrate were effective nitrogen sources for cell growth, while ammonium ions inhibited growth of these gametophytes. |
关键词 | Microprojectile Bombardment Bubble-column Bioreactor Laminaria Japonica Gametophytes Human Acidic Fibroblast Growth Factor |
学科领域 | Biotechnology & Applied Microbiology ; Plant Sciences |
DOI | 10.1007/s11240-009-9523-0 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000265942400004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/2896 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Jinan Univ, Biopharmaceut Res & Dev Ctr, Guangzhou 510632, Guangdong, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Deng, Xiangyuan,Qin, Song,Zhang, Qun,et al. Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2009,97(3):253-261. |
APA | Deng, Xiangyuan,Qin, Song,Zhang, Qun,Jiang, Peng,Cui, Yulin,&Li, Xiaokun.(2009).Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor.PLANT CELL TISSUE AND ORGAN CULTURE,97(3),253-261. |
MLA | Deng, Xiangyuan,et al."Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor".PLANT CELL TISSUE AND ORGAN CULTURE 97.3(2009):253-261. |
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