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Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor
Deng, Xiangyuan1,2; Qin, Song1; Zhang, Qun1,2; Jiang, Peng1; Cui, Yulin1,2; Li, Xiaokun3
2009-06-01
发表期刊PLANT CELL TISSUE AND ORGAN CULTURE
ISSN0167-6857
卷号97期号:3页码:253-261
文章类型Article
摘要A human acidic fibroblast growth factor gene, hafgf, was successfully transferred into Laminaria japonica (kelp) gametophytes via microprojectile bombardment using the biolistic PDS-1000/He gene gun. Following phosphinothricin screening, PCR detection and Southern blot analysis, transgenic L. japonica gametophytes were cultivated in an illuminated bubble-column bioreactor to optimize growth conditions. A maximal final dry cell density of 1,695 mg l(-1) was obtained in a batch culture having an initial dry cell density of 129.75 mg l(-1). This was achieved using an aeration rate of 1.08 l air min(-1) l(-1) culture in a medium containing 1.5 mM inorganic nitrate and 0.15 mM phosphate. In addition, the relationship between different nitrogen sources and growth of transgenic gametophytes indicated that both urea and sodium nitrate were effective nitrogen sources for cell growth, while ammonium ions inhibited growth of these gametophytes.; A human acidic fibroblast growth factor gene, hafgf, was successfully transferred into Laminaria japonica (kelp) gametophytes via microprojectile bombardment using the biolistic PDS-1000/He gene gun. Following phosphinothricin screening, PCR detection and Southern blot analysis, transgenic L. japonica gametophytes were cultivated in an illuminated bubble-column bioreactor to optimize growth conditions. A maximal final dry cell density of 1,695 mg l(-1) was obtained in a batch culture having an initial dry cell density of 129.75 mg l(-1). This was achieved using an aeration rate of 1.08 l air min(-1) l(-1) culture in a medium containing 1.5 mM inorganic nitrate and 0.15 mM phosphate. In addition, the relationship between different nitrogen sources and growth of transgenic gametophytes indicated that both urea and sodium nitrate were effective nitrogen sources for cell growth, while ammonium ions inhibited growth of these gametophytes.
关键词Microprojectile Bombardment Bubble-column Bioreactor Laminaria Japonica Gametophytes Human Acidic Fibroblast Growth Factor
学科领域Biotechnology & Applied Microbiology ; Plant Sciences
DOI10.1007/s11240-009-9523-0
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000265942400004
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/2896
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Jinan Univ, Biopharmaceut Res & Dev Ctr, Guangzhou 510632, Guangdong, Peoples R China
第一作者单位中国科学院海洋研究所
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Deng, Xiangyuan,Qin, Song,Zhang, Qun,et al. Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2009,97(3):253-261.
APA Deng, Xiangyuan,Qin, Song,Zhang, Qun,Jiang, Peng,Cui, Yulin,&Li, Xiaokun.(2009).Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor.PLANT CELL TISSUE AND ORGAN CULTURE,97(3),253-261.
MLA Deng, Xiangyuan,et al."Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor".PLANT CELL TISSUE AND ORGAN CULTURE 97.3(2009):253-261.
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