Pretreatment Method for Nitrogen Isotopic Analysis of Nitrate in Seawater

	Pretreatment Method for Nitrogen Isotopic Analysis of Nitrate in Seawater
	Liu Xiu-Juan1,2 ; Yu Zhi-Ming1 ; Song Xiu-Xian1 ; Cao Xi-Hua1
	2009-05-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	37 期号:5 页码:643-647
文章类型	Article
摘要	A suitable method for the pretreutment of dissolved nitrate samples in seawaters for nitrogen isotopic analysis was established. First, the seawater samples were processed by removing nitrite and amonium. Then Devard's alloy was added in sample for conversion of dissolved nitrate to ammonium. The sample was distilled, and then the ammonium condensate was collected with zeolite. after distillation, the collected condensate was filtered and prepared for determining nitropic values. Some tests of the method were conducted. The distillation condition, the influence of salinity on nitrogen isotopic analysis, absorption of ammonium onto zeolite and an improved method on a large volume of seawater were discussed in this study. The results showed that the distillation step had an average recovery of (104.9 +/- 4.2) % (n = 6) when distillating every 300 mL aliquot of the sample under a strong alkaline condition with 0.5 g devard's alloy and a distillation time of 30 min. The nitrogen isotopic fractionation decreased markedly when salinity was increased from 0% to 0.5%; further increase(1% - 3.5%) showed little effect. The adsorption rate of ammonium onto zeolite had a high yield of (95.96 +/- 1.08) % (n = 6) in average. An improved collection method was used to process a large volume of seawater with several distillations, and had good effect on analysis. The method had been applied to analyze water samples collected from Changjiang estuary. The analytical results indicate that the method is suitable for delta(15)N analysis of dissolved nitrate in seawaters. The present method could provide valuable information about the source and cycle mechanism of dissolved nitrogen in estuary waters.; A suitable method for the pretreutment of dissolved nitrate samples in seawaters for nitrogen isotopic analysis was established. First, the seawater samples were processed by removing nitrite and amonium. Then Devard's alloy was added in sample for conversion of dissolved nitrate to ammonium. The sample was distilled, and then the ammonium condensate was collected with zeolite. after distillation, the collected condensate was filtered and prepared for determining nitropic values. Some tests of the method were conducted. The distillation condition, the influence of salinity on nitrogen isotopic analysis, absorption of ammonium onto zeolite and an improved method on a large volume of seawater were discussed in this study. The results showed that the distillation step had an average recovery of (104.9 +/- 4.2) % (n = 6) when distillating every 300 mL aliquot of the sample under a strong alkaline condition with 0.5 g devard's alloy and a distillation time of 30 min. The nitrogen isotopic fractionation decreased markedly when salinity was increased from 0% to 0.5%; further increase(1% - 3.5%) showed little effect. The adsorption rate of ammonium onto zeolite had a high yield of (95.96 +/- 1.08) % (n = 6) in average. An improved collection method was used to process a large volume of seawater with several distillations, and had good effect on analysis. The method had been applied to analyze water samples collected from Changjiang estuary. The analytical results indicate that the method is suitable for delta N-15 analysis of dissolved nitrate in seawaters. The present method could provide valuable information about the source and cycle mechanism of dissolved nitrogen in estuary waters.
关键词	Seawater System Dissolved Nitrate Stable Nitrogen Isotopic Analysis Preparation Method
学科领域	Chemistry, Analytical
收录类别	SCI
语种	英语
WOS记录号	WOS:000266385500003
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2618
专题	海洋生态与环境科学重点实验室文献信息中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu Xiu-Juan,Yu Zhi-Ming,Song Xiu-Xian,et al. Pretreatment Method for Nitrogen Isotopic Analysis of Nitrate in Seawater[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(5):643-647.
APA	Liu Xiu-Juan,Yu Zhi-Ming,Song Xiu-Xian,&Cao Xi-Hua.(2009).Pretreatment Method for Nitrogen Isotopic Analysis of Nitrate in Seawater.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(5),643-647.
MLA	Liu Xiu-Juan,et al."Pretreatment Method for Nitrogen Isotopic Analysis of Nitrate in Seawater".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.5(2009):643-647.

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