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| Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes | |
| Cao Xuan1,2; Yu Jing-Jing2; Wang Geng3; Yu Zhen-Hua2,4; Yang Huang-Hao2; Xu Yuan-Yuan5; Sun Gui-Fan5; Wang Xiao-Ru2 | |
| 2009 | |
| 发表期刊 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| ISSN | 0253-3820 |
| 卷号 | 37期号:1页码:19-24 |
| 文章类型 | Article |
| 摘要 | The effects of direct sampling and three digestion methods were investigated on the determination of arsenic in Chang liver hepatocytes after ultrasonic disintegration were investigated. The results showed that the efficiency of microwave digestion and obturator digestion was better than cold digestion and direct sampling. The day precision (present as RSD) of microwave digestion and obturator digestion were 2.1% and 1.2% the inter-day precision were 1.2% and 2.0%, respectively. The spike recovery for the total As in the sample is 95.7% - 108.1%. The As detection limits with these four sample treatment methods (including direct sampling) were 0.74 - 0.93 mu g/L. In addition, arsenic speciation in Chang liver hepatocytes was also analyzed using the hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry. The experimental results indicated that dimethylarsinic acid (DMA) and an intermediate metabolite of DMA were found lit Chang liver hepatocytes besides inorganic arsenic (As(III) and As(V)).; The effects of direct sampling and three digestion methods were investigated on the determination of arsenic in Chang liver hepatocytes after ultrasonic disintegration were investigated. The results showed that the efficiency of microwave digestion and obturator digestion was better than cold digestion and direct sampling. The day precision (present as RSD) of microwave digestion and obturator digestion were 2.1% and 1.2% the inter-day precision were 1.2% and 2.0%, respectively. The spike recovery for the total As in the sample is 95.7% - 108.1%. The As detection limits with these four sample treatment methods (including direct sampling) were 0.74 - 0.93 mu g/L. In addition, arsenic speciation in Chang liver hepatocytes was also analyzed using the hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry. The experimental results indicated that dimethylarsinic acid (DMA) and an intermediate metabolite of DMA were found lit Chang liver hepatocytes besides inorganic arsenic (As(III) and As(V)). |
| 关键词 | Cell Digestion High Performance Liquid Chromatography-inductively Coupled Plasma-mass Sepetrometry Chang Liver Hepatocytes Arsenic Speciation |
| 学科领域 | Chemistry, Analytical |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265116600004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/2592 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266003, Peoples R China 2.First Inst Oceanog State Ocean Adm, Qingdao 266061, Peoples R China 3.Shandong Univ, Coll Chem & Chem Engn, Jinan 250100, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 5.China Med Univ, Coll Publ Hlth, Shenyang 110001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao Xuan,Yu Jing-Jing,Wang Geng,et al. Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(1):19-24. |
| APA | Cao Xuan.,Yu Jing-Jing.,Wang Geng.,Yu Zhen-Hua.,Yang Huang-Hao.,...&Wang Xiao-Ru.(2009).Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(1),19-24. |
| MLA | Cao Xuan,et al."Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.1(2009):19-24. |
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