Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls

doi:10.1016/j.algal.2015.02.002

IOCAS-IR > 实验海洋生物学重点实验室

	Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls
	Wei, Yifan 1,2; Niu, Jianfeng 1; Huan, Li1,2 ; Huang, Aiyou 1; He, Linwen 1; Wang, Guangce 1
	2015-03-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷号	8 页码:135-139
文章类型	Article
摘要	Cell penetrating peptides (CPPs) are effective molecular tools that can deliver various exogenous cargoes into different types of cells. However, no study to date has evaluated the use of CPPs to import nucleic acid substances into microalgae, which would be advantageous for numerous research and commercial applications. In this study, we tested the ability of nona-(L)-arginine (R9) to conduct CPP-mediated double-stranded RNA (dsRNA) translocations into four microalgal species (Chlamydomonas reinhardtii, Chlorella vulgaris, Phaeodactylum tricornutum, and Dunaliella salina). R9 successfully transported dsRNA into D. salina, whereas dsRNA internalizations were not detected in the other three algal species. Calcofluor white staining showed that the cell wall structure of D. salina differs from that of the other three species. These results indicate that the efficiency of CPP-mediated dsRNA transductions is closely related to algal cell wall structures, which vary in their ability to block entry of the CPP/ dsRNA complex. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Cpps Microalgae Cell Wall Photosynthesis
DOI	10.1016/j.algal.2015.02.002
收录类别	SCI
语种	英语
WOS记录号	WOS:000352274800020
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25462
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wei, Yifan,Niu, Jianfeng,Huan, Li,et al. Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2015,8:135-139.
APA	Wei, Yifan,Niu, Jianfeng,Huan, Li,Huang, Aiyou,He, Linwen,&Wang, Guangce.(2015).Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,8,135-139.
MLA	Wei, Yifan,et al."Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 8(2015):135-139.

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