Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia

doi:10.3390/md13031360

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	Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia
	Zhang, Wenjing1,2 ; Jin, Weihua 1; Sun, Delin 3; Zhao, Luyu 3; Wang, Jing1,4 ; Duan, Delin 1; Zhang, Quanbin 1
	2015-03-01
发表期刊	MARINE DRUGS
卷号	13 期号:3 页码:1360-1374
文章类型	Article
摘要	Two polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water, respectively. Composition analysis showed that these polysaccharides predominantly consisted of fucose, with galactose, mannose and glucuronic acid as minor components. After degradation and partial desulfation, electrospray ionization mass spectrometry (ESI-MS) was performed, which showed that the polysaccharides consisted of sulfated fucooligosaccharides, sulfated galactofucooligosaccharides and methyl glycosides of mono-sulfated/multi-sulfated fucooligosaccharides. The structures of the oligomeric fragments were further characterized by electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS2 and ESI-CID-MS3). Moreover, the activity of KCA and KCW against the hemolytic activity of both the classical and alternative complement pathways was determined. The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity. In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity. Moreover, the desulfated fractions (ds-DKCA and ds-DKCW) showed less or no activity, which confirmed that sulfate was important for activity. In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway.
DOI	10.3390/md13031360
收录类别	SCI
语种	英语
WOS记录号	WOS:000351930500015
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25447
专题	实验海洋生物学重点实验室海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 3.Heze Juxinyuan Food Co Ltd, Heze 274400, Peoples R China 4.Chinese Acad Sci, Nantong Branch, Inst Oceanol, Nantong 226006, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Wenjing,Jin, Weihua,Sun, Delin,et al. Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia[J]. MARINE DRUGS,2015,13(3):1360-1374.
APA	Zhang, Wenjing.,Jin, Weihua.,Sun, Delin.,Zhao, Luyu.,Wang, Jing.,...&Zhang, Quanbin.(2015).Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia.MARINE DRUGS,13(3),1360-1374.
MLA	Zhang, Wenjing,et al."Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia".MARINE DRUGS 13.3(2015):1360-1374.

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