Molecular characterization of the cathepsin B of turbot (Scophthalmus maximus)

doi:10.1007/s10695-014-9998-4

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	Molecular characterization of the cathepsin B of turbot (Scophthalmus maximus)
	Zhou, Ze-jun1,2 ; Qiu, Reng 1,2,3; Zhang, Jian 1
	2015-04-01
发表期刊	FISH PHYSIOLOGY AND BIOCHEMISTRY
卷号	41 期号:2 页码:473-483
文章类型	Article
摘要	Cathepsin B is an enzymatic protein belonging to the peptidase C1 family. It is involved in diverse physiological and pathological functions that include immune response. In this study, we identified and characterized a cathepsin B homolog (SmCatB) from turbot (Scophthalmus maximus). SmCatB is composed of 330 amino acid residues and possesses typical domain architecture of cathepsin B, which contains a propeptide region and a cysteine protease domain, and the latter processes four conserved residues (Q101, C107, H277, and N297) in the active site. SmCatB shares 80.6-87.6 % overall sequence identities with the cathepsin B of a number of teleost. SmCatB expression was detected in a wide range of tissues and upregulated by bacterial infection in a time-dependent manner. Recombinant SmCatB (rSmCatB-WT) purified from Escherichia coli exhibited apparent protease activity, which was optimal at 50 degrees C and pH 5.5. Compared to rSmCatB-WT, the mutant proteins rSmCatB-C107S, rSmCatB-H277A, and rSmCatB-N297A, which bear C107S, H277A, and N297A mutations, respectively, were significantly reduced in protease activity, with the highest reduction observed with rSmCatB-N297A. These results indicate that SmCatB is a bioactive protease that depends on the conserved structural features and that SmCatB is involved in pathogen-induced immune response.
关键词	Cathepsin b Scophthalmus Maximus Expression Cysteine Protease
DOI	10.1007/s10695-014-9998-4
收录类别	SCI
语种	英语
WOS记录号	WOS:000351506900013
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25441
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Nanyang Normal Univ, China UK NYNU RRes Joint Lab Insect Biol, Nanyang 473061, Henan, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhou, Ze-jun,Qiu, Reng,Zhang, Jian. Molecular characterization of the cathepsin B of turbot (Scophthalmus maximus)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(2):473-483.
APA	Zhou, Ze-jun,Qiu, Reng,&Zhang, Jian.(2015).Molecular characterization of the cathepsin B of turbot (Scophthalmus maximus).FISH PHYSIOLOGY AND BIOCHEMISTRY,41(2),473-483.
MLA	Zhou, Ze-jun,et al."Molecular characterization of the cathepsin B of turbot (Scophthalmus maximus)".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.2(2015):473-483.

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