| Effect of the addition of six antioxidants on sperm motility, membrane integrity and mitochondrial function in red seabream (Pagrus major) sperm cryopreservation | |
Liu, Qinghua1; Wang, Xueying1,2 ; Wang, Wenqi3; Zhang, Xuelei3; Xu, Shihong1; Ma, Daoyuan1; Xiao, Zhizhong1; Xiao, Yongshuang1; Li, Jun1
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| 2015-04-01 | |
| 发表期刊 | FISH PHYSIOLOGY AND BIOCHEMISTRY
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| 卷号 | 41期号:2页码:413-422 |
| 文章类型 | Article |
| 摘要 | The present study was to evaluate the effects of six antioxidants on frozen-thawed sperm motility, viability, membrane integrity and mitochondrial function in red seabream (Pagrus major) by computer-assisted sperm analysis system and flow cytometry, respectively. All the parameters tested in this study were determined using one-way ANOVA and identified using the SNK test (P < 0.05). The results demonstrated that on the first day, the highest motility and longevity occurred in 100 mM trehalose (78.34 +/- 3.41 %, 29 +/- 4.00 days) and 50 mM taurine (77.46 +/- 1.54 %, 29.33 +/- 4.04 days), followed by 25 mM vitamin C (79.03 +/- 5.37 %, 17 +/- 1.00 days), 25 mM vitamin E (69.64 +/- 1.64 %, 27.67 +/- 1.53 days) and 25 mM vitamin A (78.89 +/- 2.81 %, 9.33 +/- 1.53 days), which were all higher than frozen-thawed sperm without antioxidant (control) (66.80 +/- 5.55, 5.67 +/- 1.15 days). Especially, the percentages of class A sperm with the addition of 100 mM trehalose (40.39 +/- 5.20 %) and 50 mM taurine (37.78 +/- 3.22 %) were significantly improved compared to the control (19.63 +/- 5.44 %). The viability of all groups on the third and sixth day showed a similar trend. Moreover, during the 4 degrees C storage process, the decrease of frozen-thawed sperm motility was closely associated with the decrease in membrane integrity and mitochondrial function. In conclusion, the present study indicated that antioxidant (100 mM trehalose and 50 mM taurine) provided the most pronounced protective effect in improving frozen-thawed quality of red seabream sperm. The addition of antioxidant may be capable of scavenging the ROS generated during the cryopreservation process and 4 degrees C storage. |
| 关键词 | Red Seabream Cryopreservation Sperm Antioxidants |
| DOI | 10.1007/s10695-014-9993-9 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351506900008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/25438 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266109, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 3.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Qinghua,Wang, Xueying,Wang, Wenqi,et al. Effect of the addition of six antioxidants on sperm motility, membrane integrity and mitochondrial function in red seabream (Pagrus major) sperm cryopreservation[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(2):413-422. |
| APA | Liu, Qinghua.,Wang, Xueying.,Wang, Wenqi.,Zhang, Xuelei.,Xu, Shihong.,...&Li, Jun.(2015).Effect of the addition of six antioxidants on sperm motility, membrane integrity and mitochondrial function in red seabream (Pagrus major) sperm cryopreservation.FISH PHYSIOLOGY AND BIOCHEMISTRY,41(2),413-422. |
| MLA | Liu, Qinghua,et al."Effect of the addition of six antioxidants on sperm motility, membrane integrity and mitochondrial function in red seabream (Pagrus major) sperm cryopreservation".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.2(2015):413-422. |
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