| Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate | |
Wang Xueqin1,2; Xing Ronge1 ; Liu Song1; Yu Huahua1; Li Kecheng1; Chen Zuoyuan3; Li Pengcheng1
| |
| 2015 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 卷号 | 33期号:1页码:159-168 |
| 文章类型 | Article |
| 摘要 | Mackerel (Pneumatophorus japonicus) proteins were hydrolyzed by five proteases: trypsin, papain, neutrase, acid protease, and flavourzyme. The hydrolysate treated by neutrase exhibited the highest antioxidant activity. Response surface methodology (RSM) was employed to optimize the hydrolysis conditions in an effort to obtain a mackerel protein hydrolysate (MPH) with the highest DPPH radical scavenging activity. The MPH was fractioned using a series of ultrafiltration membranes and five fractions, namely, MPH-I (>10 kDa), MPH-II (10-2.5 kDa), MPH-III (1-2.5 kDa), MPH-IV (0.4-1 kDa), and MPH-V (below 0.4 kDa), were obtained. DPPH radical scavenging activity, reducing power, hydroxyl radical scavenging activity, and the lipid peroxidation inhibition capability of these fractions were evaluated. The fractions in molecular weights <2.5 kDa (MPH-III, MPH-IV, and MPH-V), which occupied 93.4% of the total fractions, showed the strongest antioxidant activity; and the antioxidant activities of the three fractions are similar to each other. Using SP Sephadex C-25 and Sephadex G-25 columns, eight fractions were obtained from the MPH (<2.5 kDa). The isolated peptide I (1 664 kDa) displayed the highest DPPH radical scavenging activity. Therefore, MPH is a potential source of antioxidant peptides. |
| 关键词 | Mackerel Protein Antioxidant Activity Fraction Of Hydrolysate Ultrafiltration Purification |
| DOI | 10.1007/s00343-014-3369-y |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351156200019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/25431 |
| 专题 | 海洋生物技术研发中心 海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Univ, Coll Med, Affiliated Hosp, Dept Cardiol, Qingdao 266003, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang Xueqin,Xing Ronge,Liu Song,et al. Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(1):159-168. |
| APA | Wang Xueqin.,Xing Ronge.,Liu Song.,Yu Huahua.,Li Kecheng.,...&Li Pengcheng.(2015).Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(1),159-168. |
| MLA | Wang Xueqin,et al."Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.1(2015):159-168. |
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