| Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature | |
Liu Yifan1,2; Ma Daoyuan1; Xiao Zhizhong1; Xu Shihong1; Wang Yanfeng1; Wang Yufu1,2 ; Xiao Yongshuang1,3; Song Zongcheng4; Teng Zhaojun5; Liu Qinghua1; Li Jun1
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| 2015 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 卷号 | 33期号:1页码:11-19 |
| 文章类型 | Article |
| 摘要 | High temperature influences the homeostasis of fish. We investigated the effects of elevated temperature on tissues of Japanese flounder (Paralichthys olivaceus) by analyzing the histology and heat shock protein 70 (hsp70) expression of fish reared in warm conditions. In this study, temperature was increased at 1 +/- 0.5 degrees C/day starting at 24 +/- 0.5 degrees C, and was kept at that temperature for 5 days before the next rise. After raising temperature at the rate up to 32 +/- 0.5 degrees C, tissue samples from midgut, spleen, stomach, liver, muscle, gill, heart, trunk kidney and brain were collected for histological analysis and mRNA assay. Almost all the tissues showed changes in morphological structure and hsp70 level at 32 +/- 0.5 degrees C. Histological assessment of the tissues indicated that the gill had the most serious damage, including highly severe epithelial lifting and edema, curved tips and hyperemia at the ending of the lamellars, desquamation and necrosis. The next most severe damage was found in liver and kidney. The hsp70 levels in all the tissues first increased and then decreased. The gut, stomach, muscle, heart, and brain had the highest expressions in 6 h, whereas the spleen, liver, gill and kidney had the highest expressions in 2 h. Therefore, tissues with the most significant lesions (especially gill and liver) responded much earlier (2 h) in hsp70 expression than other tissues, and these tissues demonstrated the most marked histological disruption and elevated mRNA levels, making them ideal candidates for further studies on the thermal physiology of this species. |
| 关键词 | Temperature Paralichthys Olivaceus Histology Hsp70 Tissue Expression |
| DOI | 10.1007/s00343-015-4028-7 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351156200002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/25411 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Nantong Branch, Nantong 226019, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China 5.Rizhao Proliferat Stn Marine Aquat Resources, Rizhao 276805, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu Yifan,Ma Daoyuan,Xiao Zhizhong,et al. Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(1):11-19. |
| APA | Liu Yifan.,Ma Daoyuan.,Xiao Zhizhong.,Xu Shihong.,Wang Yanfeng.,...&Li Jun.(2015).Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(1),11-19. |
| MLA | Liu Yifan,et al."Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.1(2015):11-19. |
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